{"product_id":"cst-88701s","title":"CST,  88701S, ORF1p (D3W9O) Rabbit Monoclonal Antibody","description":"Monoclonal Antibody for studying L1RE1. Validated for Western Blotting,Simple Western™. Available in 2 sizes. Highly specific and rigorously validated in-house, ORF1p (D3W9O) Rabbit Monoclonal Antibody (CST #88701) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nWestern Blotting: 1:1000\nSimple Western™: 1:10 - 1:50\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nORF1p (D3W9O) Rabbit Monoclonal Antibody recognizes endogenous levels of total ORF1p protein.\nSpecies Reactivity: Human\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nMonoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly162 of human ORF1p protein.\n\u003cb\u003eBackground\u003c\/b\u003e\nLINE-1, also known as L1, is a non-long terminal repeat (non-LTR) retrotransposon with hundreds of thousands of copies in the human genome (1, 2). Like all non-LTRs, L1 replicates by target-primed reverse transcription (TPRT) (3). The L1 retrotransposon encodes two proteins critical to this process: ORF1p and ORF2p. ORF2p contributes to endonuclease and reverse transcriptase activity, while ORF1p acts as a nucleic acid chaperone that binds RNA (4-8). Many types of cancers have been shown to have L1 insertions within tumor suppressor genes, disrupting their expression and contributing to tumorigenesis (9). While normally silenced, ORF1p is expressed in many types of cancers (9-12). Recently, it has been found that ORF1p can become phosphorylated and that this event impacts its ability to promote retrotransposition. This creates a novel dynamic to which the host could actively affect L1 replication through various signaling cascades (13).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nL1ORF1p; L1RE1; LINE retrotransposable element 1; LINE-1 retrotransposable element ORF1 protein; LINE1 retrotransposable element 1; LORF1; LRE1\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H\nSENSITIVITY: Endogenous\nMW (kDa): 42\nSource\/Isotype: Rabbit IgG","brand":"CST","offers":[{"title":"Default Title","offer_id":46799983509673,"sku":"88701S","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-88701s","provider":"Iright","version":"1.0","type":"link"}