{"product_id":"cst-88829s","title":"CST,  88829S, CD8 alpha (RPA-T8) Mouse Monoclonal Antibody (PE Conjugate)","description":"Monoclonal Antibody for studying CD8A. Validated for Flow Cytometry (Fixed\/Permeabilized),Flow Cytometry (Live). Highly specific and rigorously validated in-house, CD8 alpha (RPA-T8) Mouse Monoclonal Antibody (PE Conjugate) (CST #88829) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nFlow Cytometry (Fixed\/Permeabilized): 1:20\nFlow Cytometry (Live): 1:20\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM NaH2PO4, 150 mM NaCl, 0.09% NaN3, 0.1% gelatin, pH 7.2. This product is stable for 12 months when stored at 4ºC. Do not aliquot the antibody. Protect from light. Do not freeze.\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Flow Cytometry (Fixed\/Permeabilized), Flow Cytometry (Live)\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nCD8 alpha (RPA-T8) Mouse Monoclonal Antibody (PE Conjugate) recognizes endogenous levels of total CD8α protein. This antibody conjugate detects an epitope within the extracellular domain.\nSpecies Reactivity: Human\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nThis monoclonal antibody was purified from tissue culture supernatant via affinity chromatography. The purified antibody was conjugated under optimal conditions, with unreacted dye removed from the preparation.\n\u003cb\u003eBackground\u003c\/b\u003e\nCluster of Differentiation 8 (CD8) is a disulphide-linked heterodimer consisting of the unrelated α and β subunits. Each subunit is a glycoprotein composed of a single extracellular Ig-like domain, a polypeptide linker, a transmembrane part and a short cytoplasmic tail. On T cells, CD8 is the coreceptor for the T cell receptor (TCR), and these two distinct structures recognize the Antigen-Major Histocompatibility Complex (MHC). Specifically, the Ig-like domain of CD8α interacts with the α3-domain of the MHC class I molecule. CD8 ensures specificity of the TCR-antigen interaction, prolongs the contact between the T cell and the antigen presenting cell, and the α chain recruits the tyrosine kinase Lck, which is essential for T cell activation (1). The RPA-T8 antibody is widely used as a phenotypic marker for CD8 on cytotoxic T cells and thymocytes (2,3), as well as on certain cell types that do not express the TCR, including some NK cells (4).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nCD8; CD8 antigen alpha polypeptide; CD8 antigen, alpha polypeptide (p32); CD8A; CD8a molecule; Leu2; Leu2 T-lymphocyte antigen; MAL; OKT8 T-cell antigen; p32; T cell co-receptor; T-cell antigen Leu2; T-cell surface glycoprotein CD8 alpha chain; T-lymphocyte differentiation antigen T8\/Leu-2; T8 T-cell antigen\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H\nSENSITIVITY: Endogenous\nSource\/Isotype: Mouse IgG1","brand":"CST","offers":[{"title":"Default Title","offer_id":46800552263849,"sku":"88829S","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-88829s","provider":"Iright","version":"1.0","type":"link"}