{"product_id":"cst-89036sf","title":"CST,  89036SF, Blimp-1\/PRDI-BF1 (C14A4) Rabbit Monoclonal Antibody (BSA and Azide Free)","description":"Monoclonal Antibody for studying PRD1. Validated for Western Blotting,Immunofluorescence (Immunocytochemistry),Flow Cytometry (Fixed\/Permeabilized). Highly specific and rigorously validated in-house, Blimp-1\/PRDI-BF1 (C14A4) Rabbit Monoclonal Antibody (BSA and Azide Free) (CST #89036) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nThis product is the carrier free version of product #9115. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol. This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT\u0026amp;RUN or CUT\u0026amp;Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us . Optimal dilutions\/concentrations should be determined by the end user. BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity.\n\u003cb\u003eFormulation\u003c\/b\u003e\nSupplied in 1X PBS (10 mM Na 2 HPO 4 , 3 mM KCl, 2 mM KH 2 PO 4 , and 140 mM NaCl (pH 7.8)). BSA and Azide Free. For standard formulation of this product see product # 9115 .\n\u003cb\u003eStorage\u003c\/b\u003e\nStore at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze\/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance.\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nBlimp-1\/PRDI-BF1 (C14A4) Rabbit Monoclonal Antibody (BSA and Azide Free) detects endogenous levels of total Blimp-1\/PRDI-BF1 protein.\nSpecies Reactivity: Human, Mouse\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nMonoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val90 of human PRDI-BF1 isoform 2.\n\u003cb\u003eBackground\u003c\/b\u003e\nBlimp-1 (B lymphocyte-induced maturation protein) is a nuclear zinc-finger containing transcriptional repressor that is considered a master regulator of terminal B cell development (1). The human homolog, PRDI-BF1, was identified by its ability to bind to the PRDI element on the IFN-β promoter and can inhibit virus-mediated IFN-β production (2). Expression of Blimp-1 is sufficient to drive terminal differentiation of BCL1 lymphoma cells into antibody secreting plasma cells, increasing the expression of the cell surface marker Syndecan-1 (1). In the B cell lineage, Blimp-1 is specifically expressed in antibody-secreting cells including activated B and plasma cells. In addition, Blimp-1 has been found during macrophage differentiation (3) and in a subset of T cells (4,5), suggesting it may play a wider role in homeostasis and differentiation (6). Mechanistically, Blimp-1 is thought to act by recruiting chromatin-modifying enzymes including histone deacetylases (7) and methyltransferases (8,9). Target genes of Blimp-1 transcriptional repression with potential roles in differentiation include c-Myc (10), CIITA (11), Pax5 (12), Spi-B, and Id3 (13).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nB-lymphocyte-induced maturation protein 1; Beta-interferon gene positive regulatory domain I-binding factor; beta-interferon gene positive-regulatory domain I binding factor; BLIMP-1; BLIMP1; MGC118922; MGC118923; MGC118924; MGC118925; Positive regulatory domain I-binding factor 1; PR domain 1; PR domain containing 1, with ZNF domain; PR domain zinc finger protein 1; PR domain-containing protein 1; PR-domain zinc finger protein 1; PR\/SET domain 1; PRDI-BF1; PRDI-binding factor 1; PRDI-binding factor-1; PRDM1\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H M\nSENSITIVITY: Endogenous\nMW (kDa): 95-100\nSource\/Isotype: Rabbit IgG","brand":"CST","offers":[{"title":"Default Title","offer_id":46799988195497,"sku":"89036SF","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-89036sf","provider":"Iright","version":"1.0","type":"link"}