{"product_id":"cst-89261w2","title":"CST,  89261W2, Bak (D4E4) Rabbit Monoclonal Antibody (SignalFlex™ mFluor™ Violet 510 Conjugate)","description":"Monoclonal Antibody for studying Bak1. Highly specific and rigorously validated in-house, Bak (D4E4) Rabbit Monoclonal Antibody (SignalFlex\u003csup\u003e™\u003c\/sup\u003e mFluor\u003csup\u003e™\u003c\/sup\u003e Violet 510 Conjugate) (CST #89261) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nSignalFlex™ conjugates are produced using highly validated Cell Signaling Technology ® primary antibodies and conjugation methods that have been rigorously tested, ensuring high-quality conjugates and lot-to-lot consistency. These conjugates are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity. However, they are not tested on specific assays. Optimal dilutions\/concentrations should be determined by the end user. When performing flow cytometry, we recommend using an isotype control conjugate at the same concentration as the antibody conjugate.\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in PBS (pH 7.2), less than 0.1% sodium azide, and 2 mg\/mL BSA. Store at 4°C. Do not aliquot the antibody. Protect from light. Do not freeze.\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nBak (D4E4) Rabbit mAb (SignalFlex™ mFluor™ Violet 510 Conjugate) recognizes endogenous levels of total Bak protein.\nSpecies Reactivity: Human, Mouse, Rat, Monkey\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nMonoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly75 of human Bak protein.\n\u003cb\u003eBackground\u003c\/b\u003e\nBak is a proapoptotic member of the Bcl-2 family (1). This protein is located on the outer membrane of mitochondria and is an essential component for transduction of apoptotic signals through the mitochondrial pathway (2,3). Upon apoptotic stimulation, an upstream stimulator like truncated BID (tBID) induces conformational changes in Bak to form oligomer channels in the mitochondrial membrane for cytochrome c release. The release of cytochrome c to the cytosol activates the caspase-9 pathway and eventually leads to cell death (4,5).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nApoptosis regulator BAK; BAK; BAK-LIKE; BAK1; Bcl-2 homologous antagonist\/killer; Bcl-2-like protein 7; BCL2 antagonist\/killer 1; BCL2-antagonist\/killer 1; Bcl2-L-7; BCL2-like 7 protein; BCL2L7; CDN1; MGC117255; MGC3887; pro-apoptotic protein BAK\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H M R Mk\nSENSITIVITY: Endogenous\nSource\/Isotype: Rabbit IgG","brand":"CST","offers":[{"title":"Default Title","offer_id":46800553181353,"sku":"89261W2","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-89261w2","provider":"Iright","version":"1.0","type":"link"}