{"product_id":"cst-89289sf","title":"CST,  89289SF, Connexin 43 (E7N2R) Rabbit Monoclonal Antibody (BSA and Azide Free)","description":"Monoclonal Antibody for studying Connexin-43. Validated for Western Blotting,Immunohistochemistry (Paraffin),Immunofluorescence (Immunocytochemistry). Highly specific and rigorously validated in-house, Connexin 43 (E7N2R) Rabbit Monoclonal Antibody (BSA and Azide Free) (CST #89289) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nThis product is the carrier free version of product #83649. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol. This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT\u0026amp;RUN or CUT\u0026amp;Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us . Optimal dilutions\/concentrations should be determined by the end user. BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity.\n\u003cb\u003eFormulation\u003c\/b\u003e\nSupplied in 1X PBS (10 mM Na 2 HPO 4 , 3 mM KCl, 2 mM KH 2 PO 4 , and 140 mM NaCl (pH 7.8)). BSA and Azide Free. For standard formulation of this product see product # 83649\n\u003cb\u003eStorage\u003c\/b\u003e\nStore at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze\/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance.\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nConnexin 43 (E7N2R) Rabbit Monoclonal Antibody (BSA and Azide Free) recognizes endogenous levels of total connexin 43 protein.\nSpecies Reactivity: Human, Mouse, Rat\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nMonoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Arg374 of human connexin 43 protein.\n\u003cb\u003eBackground\u003c\/b\u003e\nConnexin 43 (Cx43) is a member of the large family of gap junction proteins. Connexins assemble as a hexamer and are transported to the plasma membrane to create a hemichannel that can associate with hemichannels on nearby cells to create cell-to-cell channels. Clusters of these channels assemble to make gap junctions. Gap junction communication is important in development and regulation of cell growth. Phosphorylation of Cx43 is important in regulating assembly and function of gap junctions (1,2). Ser368 of Cx43 is phosphorylated by protein kinase C (PKC) after activation by phorbol esters, which decreases cell-to-cell communication (3). Src can interact with and phosphorylate Cx43 to alter gap junction communication (4,5).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nAVSD3; CMDR; connexin 43; Connexin-43; Cx43; CXA1; DFNB38; EKVP; EKVP3; Gap junction 43 kDa heart protein; Gap junction alpha-1 protein; gap junction protein alpha 1; gap junction protein, alpha 1, 43kDa; gap junction protein, alpha-like; GJA1; GJAL; HLHS1; HSS; ODDD; PPKCA\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H M R\nSENSITIVITY: Endogenous\nMW (kDa): 39, 41, 43, 44\nSource\/Isotype: Rabbit IgG","brand":"CST","offers":[{"title":"Default Title","offer_id":46799989473449,"sku":"89289SF","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-89289sf","provider":"Iright","version":"1.0","type":"link"}