{"product_id":"cst-89572s","title":"CST,  89572S, Galectin-3\/LGALS3 (E7B6R) Rabbit Monoclonal Antibody","description":"Monoclonal Antibody for studying galectin-3 mouse. Validated for WB,WB,IP,IHC,IF,IF. Available in 2 sizes. Highly specific and rigorously validated in-house, Galectin-3\/LGALS3 (E7B6R) Rabbit Monoclonal Antibody (CST #89572) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nWestern Blotting: 1:1000\nSimple Western™: 1:10 - 1:50\nImmunoprecipitation: 1:50\nImmunohistochemistry (Paraffin): 1:50 - 1:200\nImmunofluorescence (Frozen): 1:100 - 1:400\nImmunofluorescence (Immunocytochemistry): 1:800 - 1:3200\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody . For a carrier free (BSA and azide free) version of this product see product # 76015 .\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting, Immunoprecipitation, Immunohistochemistry (Paraffin), Immunofluorescence (Frozen), Immunofluorescence (Immunocytochemistry)\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nGalectin-3\/LGALS3 (E7B6R) Rabbit Monoclonal Antibody recognizes endogenous levels of total Galectin-3\/LGALS3 protein. This antibody recognizes mouse Galectin-3\/LGALS3 protein and is also reactive with human Galectin-3\/LGALS3; however, this antibody is not suggested for immunohistochemical analysis of human tissues.\nSpecies Reactivity: Human, Mouse, Rat\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nMonoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly15 of mouse Galectin-3\/LGALS3 protein.\n\u003cb\u003eBackground\u003c\/b\u003e\nGalectins are a family of β-galactose binding proteins that are characterized by an affinity for poly-N-acetyllactosamine-enriched glycoconjugates and a carbohydrate-binding site (1,2). Members of the galectin family have been implicated in a variety of biological functions, including cell adhesion (3), growth regulation (4), cytokine production (5), T cell apoptosis (6), and immune responses (7). Galectin-3\/LGALS3 is involved in several diverse biological functions. Galectin-3\/LGALS3 binds IgE (8). Galectin-3\/LGALS3 is an unusual protein in that it can be found both extracellularly and intracellularly. Intracellularly, Galectin-3\/LGALS3 can localize to the cytoplasm, nucleus, or both, depending on cell type and experimental conditions. Nuclear Galectin-3\/LGALS3 has been identified as a pre-mRNA splicing factor (9). Galectin-3\/LGALS3 production has been shown to increase during inflammation and in obesity, and the protein itself can have an inflammatory effect under certain conditions (10). Galectin-3\/LGALS3 forms a complex with α3, β1 integrin to act as a surface receptor on endothelial cells for the NG2 proteoglycan, which triggers cell motility and angiogenesis (11). In addition to these functions, Galectin-3\/LGALS3 is also a required factor for the terminal differentiation of epithelial cells (12).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\n35 kDa lectin; Carbohydrate-binding protein 35; CBP 35; Gal-3; gal3; Galactose-specific lectin 3; galect; Galectin-3; GBP; IgE-binding protein; L-3; L-34; L-34 galactoside-binding lectin; Laminin-binding protein; Lectin L-29; lectin, galactose binding, soluble 3; LEG3; Lgals3; Mac-; Mac-2; Mac-2 antigen\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H M R\nSENSITIVITY: Endogenous\nMW (kDa): 28\nSource\/Isotype: Rabbit IgG","brand":"CST","offers":[{"title":"Default Title","offer_id":46799991406761,"sku":"89572S","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-89572s","provider":"Iright","version":"1.0","type":"link"}