{"product_id":"cst-90176t","title":"CST,  90176T, CD19 (Intracellular Domain) (D4V4B) Rabbit Monoclonal Antibody","description":"Monoclonal Antibody for studying CD19. Validated for WB,IP,IHC,IHC,F. Available in 2 sizes. Highly specific and rigorously validated in-house, CD19 (Intracellular Domain) (D4V4B) Rabbit Monoclonal Antibody (CST #90176) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nWestern Blotting: 1:1000\nImmunoprecipitation: 1:100\nIHC Leica Bond: 1:800 - 1:3200\nImmunohistochemistry (Paraffin): 1:1600 - 1:6400\nFlow Cytometry (Fixed\/Permeabilized): 1:6400 - 1:12800\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 86916 .\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting, Immunoprecipitation, IHC Leica Bond, Immunohistochemistry (Paraffin), Flow Cytometry (Fixed\/Permeabilized)\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nCD19 (Intracellular Domain) (D4V4B) Rabbit Monoclonal Antibody recognizes endogenous levels of total CD19 protein.\nSpecies Reactivity: Human, Mouse, Monkey\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nMonoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu427 of human CD19 protein.\n\u003cb\u003eBackground\u003c\/b\u003e\nCD19 is a 95 kDa coreceptor which amplifies the signaling cascade in B cells (1). On the B cell surface, CD19 associates with CD21, CD81, and Leu-13 to exert its function. The cytoplasmic tail of CD19 has nine conserved tyrosine residues playing critical roles in CD19 mediated function by coupling signaling molecules to the receptor (1). After B cell receptor or CD19 ligation, Tyr531 and Tyr500 of CD19 are progressively phosphorylated. This phosphorylation enables the coupling of PI3 kinase and Src family tyrosine kinase to CD19 and activates the PI3K and Src signaling pathways (2,3). Coligation of B cell receptor and CD19 also promotes Tyr409 phosphorylation in CD19. The phosphorylation at these sites enables its binding to Vav and mediates elevated intracellular calcium response, as well as the JNK pathway (4,5).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nB-lymphocyte antigen CD19; B-lymphocyte surface antigen B4; B4; CD19; CD19 antigen; CD19 molecule; CVID3; Differentiation antigen CD19; MGC12802; T-cell surface antigen Leu-12\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H M Mk\nSENSITIVITY: Endogenous\nMW (kDa): 95\nSource\/Isotype: Rabbit IgG","brand":"CST","offers":[{"title":"Default Title","offer_id":46799995666601,"sku":"90176T","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-90176t","provider":"Iright","version":"1.0","type":"link"}