{"product_id":"cst-90336s","title":"CST,  90336S, NONO Antibody","description":"Polyclonal Antibody for studying NONO. Validated for Western Blotting,Immunofluorescence (Immunocytochemistry). Highly specific and rigorously validated in-house, NONO Antibody (CST #90336) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nWestern Blotting: 1:1000\nImmunofluorescence (Immunocytochemistry): 1:50 - 1:200\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody .\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting, Immunofluorescence (Immunocytochemistry)\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nNONO Antibody recognizes endogenous levels of total NONO protein.\nSpecies Reactivity: Human, Mouse, Rat, Monkey\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nPolyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human NONO protein. Antibodies are purified by peptide affinity chromatography.\n\u003cb\u003eBackground\u003c\/b\u003e\nNon-POU domain-containing octamer-binding protein (NONO), also referred to as p54nrb, is a DNA and RNA-binding protein that is involved in several nuclear processes. NONO binds to the conventional octamer duplex of double-stranded DNA, and can additionally bind to single-stranded DNA and RNA at a location independent of the duplex site (1). NONO belongs to the Drosophila melanogaster behavior, human splicing (DBHS) protein family along with paraspeckle protein 1 (PSPC1) and splicing factor, proline- and glutamine-rich (PSF\/SFPQ) (2). NONO, PSPC1, and PSF\/SFPQ associate together along with long nonprotein-coding RNA to form ribonucleic protein bodies referred to as paraspeckles, which play a role in gene expression and regulation (2). NONO heterodimerizes with PSF\/SFPQ to process pri-miRNA prior to presentation to the Drosha complex (3). The NONO\/PSF heterodimer can also stabilize paired DNA ends and promote DNA double-strand break repair by canonical non-homologous end rejoining (4). NONO also forms a complex with PSF\/SFPQ and DExH-box helicase 9 (DHX9) that produces a long isoform of BIN1, promoting carcinogenesis in hepatocellular carcinoma (5). In breast cancer, NONO regulates pre-mRNA splicing and binding to mRNAs of cell proliferation-related genes, regardless of hormone sensitivity (6). NONO has also been hypothesized to play a role in heart disease, and a recent study in mice showed that NONO deficiency led to heart defects by fibroblast hyperproliferation (7).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\n54 kDa nuclear RNA- and DNA-binding protein; 55 kDa nuclear protein; DNA-binding p52\/p100 complex, 52 kDa subunit; MRXS34; NMT55; non-POU domain containing octamer binding; non-POU domain containing, octamer-binding; non-POU domain-containing octamer (ATGCAAAT) binding protein; Non-POU domain-containing octamer-binding protein; NONO; NonO protein; NRB54; P54; p54(nrb); p54nrb; PPP1R114; protein phosphatase 1, regulatory subunit 114\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H M R Mk\nSENSITIVITY: Endogenous\nMW (kDa): 60\nSOURCE: Rabbit","brand":"CST","offers":[{"title":"Default Title","offer_id":46799997075625,"sku":"90336S","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-90336s","provider":"Iright","version":"1.0","type":"link"}