{"product_id":"cst-90551t","title":"CST,  90551T, Tollip (F4Y5L) Rabbit Monoclonal Antibody","description":"Monoclonal Antibody for studying Tollip. Validated for WB,IP,IF,IF,F. Available in 2 sizes. Highly specific and rigorously validated in-house, Tollip (F4Y5L) Rabbit Monoclonal Antibody (CST #90551) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nWestern Blotting: 1:1000\nImmunoprecipitation: 1:50\nImmunofluorescence (Frozen): 1:50 - 1:200\nImmunofluorescence (Immunocytochemistry): 1:50 - 1:200\nFlow Cytometry (Fixed\/Permeabilized): 1:100 - 1:400\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting, Immunoprecipitation, Immunofluorescence (Frozen), Immunofluorescence (Immunocytochemistry), Flow Cytometry (Fixed\/Permeabilized)\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nTollip (F4Y5L) Rabbit Monoclonal Antibody recognizes endogenous levels of total Tollip protein.\nSpecies Reactivity: Human, Mouse, Rat, Monkey\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nMonoclonal antibody is produced by immunizing animals with full-length recombinant human Tollip protein.\n\u003cb\u003eBackground\u003c\/b\u003e\nMembers of the Toll-like receptor (TLR) family, named for the closely related Toll receptor in , play a pivotal role in innate immune responses (1-4). TLRs recognize conserved motifs found in various pathogens and mediate defense responses (5-7). Triggering of the TLR pathway leads to the activation of NF-κB and subsequent regulation of immune and inflammatory genes (4). The TLRs and members of the IL-1 receptor family share a conserved stretch of approximately 200 amino acids known as the Toll\/Interleukin-1 receptor (TIR) domain (1). Upon activation, TLRs associate with a number of cytoplasmic adapter proteins containing TIR domains, including myeloid differentiation factor 88 (MyD88), MyD88-adapter-like\/TIR-associated protein (MAL\/TIRAP), TIR domain-containing adapter-inducing IFN-β (TRIF), and Toll-receptor-associated molecule (TRAM) (8-10). This association leads to the recruitment and activation of IRAK1 and IRAK4, which form a complex with TRAF6 to activate TAK1 and IKK (8,11-14). Activation of IKK leads to the degradation of IκB, which normally maintains NF-κB in an inactive state by sequestering it in the cytoplasm. Toll-interacting protein (Tollip) is an adaptor protein discovered to be associated with the IRAK complex and recruited to IL-1R following IL-1 stimulation (4). Overexpression of Tollip results in impaired NF-κB signaling (4). Tollip also directly associates with TLR2 and TLR4, inhibiting TLR-mediated signaling by inhibiting IRAK (5). Studies of Tollip-deficient mice suggest that it plays a role in regulating inflammatory cytokine responses to IL-1 and LPS (6).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nadapter protein; FLJ33531; IL-1RAcPIP; TOLIP; toll interacting protein; Toll-interacting protein; TOLLIP\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H M R Mk\nSENSITIVITY: Endogenous\nMW (kDa): 33\nSource\/Isotype: Rabbit IgG","brand":"CST","offers":[{"title":"Default Title","offer_id":46799999795369,"sku":"90551T","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-90551t","provider":"Iright","version":"1.0","type":"link"}