{"product_id":"cst-9095s","title":"CST,  9095S, XTT Cell Viability Kit","description":"Assay Kit for studying in the research area.\n\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Functional\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nThe XTT Cell Viability Kit detects formazan dye produced from XTT conversion by mitochondrial enzymes in cells. Because these mitochondrial enzymes are inactivated shortly after cell death, the orange colored formazan dye only appears in viable cells. This XTT Cell Viability Kit is expected to work in most cells lines. Variable with cell type and incubation time employed in the assay, 0.2-2x104 cells\/well should be sufficient for most experiments. For the best result, a cell number titration (as shown in Figure 1) is recommended.\nSpecies Reactivity: All Species Expected\n\u003cb\u003eBackground\u003c\/b\u003e\nCell viability and proliferation assays are widely used in drug discovery for the study of growth factors, cytokines, and cytotoxic agents. High throughput screening, in early drug discovery compound screening and in later drug safety and toxicity studies, requires a reliable, sensitive, and simple assay with the ability to analyze a large number of samples. Colorimetric cell viability assays using tetrazolium salt, such as MTT, XTT, WST-1, etc. were developed based on live cells reduction of tetrazolium salt into highly colored formazan compounds (1,2). In contrast to cell proliferation assays, such as radioactive thymidine or BrdU labeling of DNA in live cells followed by quantification of the incorporated thymidine (by quantifying sample radioactivity) or BrdU (using anti-BrdU antibody), the XTT assay doesn't require radioactive materials, cell fixing, or cell permeabilization. Thus, unlike alternative cellular analysis assays, cells examined in the XTT assay may be used for further analysis.\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nMTT; viability assay","brand":"CST","offers":[{"title":"Default Title","offer_id":46800802906281,"sku":"9095S","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-9095s","provider":"Iright","version":"1.0","type":"link"}