{"product_id":"cst-9121l","title":"CST,  9121L, Phospho-MEK1\/2 (Ser217\/221) Antibody","description":"Polyclonal Antibody for studying MEK2 (Ser222\/Ser226) phosphate\/MEK1 (Ser218\/Ser222) phosphate. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, Phospho-MEK1\/2 (Ser217\/221) Antibody (CST #9121) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nWestern Blotting: 1:1000\nImmunoprecipitation: 1:50\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody.\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting, Immunoprecipitation\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nPhospho-MEK1\/2 (Ser217\/221) Antibody detects endogenous levels of MEK1\/2 only when activated by phosphorylation at Ser217\/221. This antibody does not cross-react with related kinases including activated SEK (MKK4), MKK3 or MKK6. This antibody will also react with MEK1\/2 singly phosphorylated at Ser217 and singly phosphorylated at Ser221.\nSpecies Reactivity: Human, Mouse, Rat, Monkey, S. cerevisiae\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nPolyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues around Ser217\/221 of human MEK1\/2. Antibodies are purified by protein A and peptide affinity chromatography.\n\u003cb\u003eBackground\u003c\/b\u003e\nMEK1 and MEK2, also called MAPK or Erk kinases, are dual-specificity protein kinases that function in a mitogen activated protein kinase cascade controlling cell growth and differentiation (1-3). Activation of MEK1 and MEK2 occurs through phosphorylation of two serine residues at positions 217 and 221, located in the activation loop of subdomain VIII, by Raf-like molecules. MEK1\/2 is activated by a wide variety of growth factors and cytokines and also by membrane depolarization and calcium influx (1-4). Constitutively active forms of MEK1\/2 are sufficient for the transformation of NIH\/3T3 cells or the differentiation of PC-12 cells (4). MEK activates p44 and p42 MAP kinase by phosphorylating both threonine and tyrosine residues at sites located within the activation loop of kinase subdomain VIII. CST's Phospho- MEK1\/2 (Ser217\/221) Antibody selectively recognizes active MEK, i.e., only when phosphorylated at Ser217\/221, and hence is an excellent marker of MEK1\/2 activity.\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nCFC3; CFC4; Dual specificity mitogen-activated protein kinase kinase 1; Dual specificity mitogen-activated protein kinase kinase 2; ERK activator kinase 1; ERK activator kinase 2; FLJ26075; MAP kinase kinase 1; MAP kinase kinase 2; MAP2K1; MAP2K2; MAPK\/ERK kinase 1; MAPK\/ERK kinase 2; MAPKK 1; MAPKK 2; MAPKK1; MAPKK2; MEK 1; MEK 2; MEK1; MEK2; MEL; mitogen-activated protein kinase kinase 1; mitogen-activated protein kinase kinase 2; mitogen-activated protein kinase kinase 2, p45; MKK1; MKK2; MP2K1; MP2K2; PRKMK1; PRKMK2; protein kinase, mitogen-activated, kinase 1 (MAP kinase kinase 1)\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H M R Mk Sc\nSENSITIVITY: Endogenous\nMW (kDa): 45\nSOURCE: Rabbit","brand":"CST","offers":[{"title":"Default Title","offer_id":46800004841641,"sku":"9121L","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-9121l","provider":"Iright","version":"1.0","type":"link"}