{"product_id":"cst-9129l","title":"CST,  9129L, Ki-67 (D3B5) Rabbit Monoclonal Antibody","description":"Monoclonal Antibody for studying KI-67. Validated for Immunofluorescence (Frozen),Immunofluorescence (Immunocytochemistry),Flow Cytometry (Fixed\/Permeabilized). Available in 3 sizes. Highly specific and rigorously validated in-house, Ki-67 (D3B5) Rabbit Monoclonal Antibody (CST #9129) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nImmunofluorescence (Frozen): 1:400 - 1:800\nImmunofluorescence (Immunocytochemistry): 1:400 - 1:800\nFlow Cytometry (Fixed\/Permeabilized): 1:400 - 1:1600\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 34330 .\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Immunofluorescence (Frozen), Immunofluorescence (Immunocytochemistry), Flow Cytometry (Fixed\/Permeabilized)\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nKi-67 (D3B5) Rabbit Monoclonal Antibody recognizes endogenous levels of total Ki-67 protein.\nSpecies Reactivity: Human, Mouse, Rat\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nMonoclonal antibody is produced by immunizing animals with a recombinant protein fragment corresponding to residues near the central region of human Ki-67 protein.\n\u003cb\u003eBackground\u003c\/b\u003e\nKi-67, named after the location where it was discovered (Kiel University, Germany), is a nuclear nonhistone protein (1) that is universally expressed among proliferating cells and absent in quiescent cells (2). Ki-67 detects proliferating cells in G1, S, G2, and mitosis, but not in the G0 resting phase. Research studies have shown that high levels of Ki-67 are associated with poorer breast cancer survival (3). Research studies have explored the use of Ki-67, along with other markers, as potential prognostic or predictive markers in breast cancer and other malignant diseases (4).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nantigen identified by monoclonal antibody Ki-67; Antigen KI-67; Antigen Ki67; KI-67; KI67; KIA; marker of proliferation Ki-67; MIB-; MIB-1; MKI67; PPP1R105; Proliferation marker protein Ki-67; proliferation-related Ki-67 antigen; protein phosphatase 1, regulatory subunit 105\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H M R\nSENSITIVITY: Endogenous\nSource\/Isotype: Rabbit IgG","brand":"CST","offers":[{"title":"Default Title","offer_id":46800273965225,"sku":"9129L","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-9129l","provider":"Iright","version":"1.0","type":"link"}