{"product_id":"cst-91336s","title":"CST,  91336S, PTMScan® Phospho-Thr-X-Arg Motif [pT(P\/X)R] Kit","description":"PTMScan for studying in the research area.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nCells are lysed in a urea-containing buffer, cellular proteins are digested by proteases, and the resulting peptides are purified by reversed-phase solid-phase extraction. Peptides are then subjected to immunoaffinity purification using a PTMScan ® Motif Antibody conjugated to protein A agarose beads. Unbound peptides are removed through washing, and the captured PTM-containing peptides are eluted with dilute acid. Reversed-phase purification is performed on microtips to desalt and separate peptides from antibody prior to concentrating the enriched peptides for LC-MS\/MS analysis. CST recommends the use of PTMScan ® IAP Buffer #9993 included in the kit.\n\u003cb\u003eStorage\u003c\/b\u003e\nAntibody beads supplied in IAP buffer containing 50% glycerol. Store at -20°C. Do not aliquot the antibody.\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: PTMScan\n\u003cb\u003eBackground\u003c\/b\u003e\nThe MAPK and CDK families of serine\/threonine protein kinases play important roles in proliferation and cell cycle control. These kinases phosphorylate threonine or serine residues that are followed by a proline residue (1-3). MAPK phosphorylates substrates with the consensus sequence PX(S\/T)P and CDKs phosphorylate substrates containing the consensus sequence (S\/T)PXR\/K (4,5). Some signaling molecules can be regulated by phosphorylation at a specific threonine followed by an arginine or lysine at the +2 position. For example, conventional PKC isozymes phosphorylate substrates containing a serine or threonine with Arg or Lys at the -3, -2 and +2 positions (6,7). Cell Signaling Technology has developed antibodies that bind to phosphorylation sites containing the T(P\/X)R motif for discovery and proteome-wide profiling of kinase substrates.\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nTPR; TXR","brand":"CST","offers":[{"title":"Default Title","offer_id":46800797008041,"sku":"91336S","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-91336s","provider":"Iright","version":"1.0","type":"link"}