{"product_id":"cst-9149s","title":"CST,  9149S, SMG-1 (D42D5) Rabbit Monoclonal Antibody","description":"Monoclonal Antibody for studying SMG1. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, SMG-1 (D42D5) Rabbit Monoclonal Antibody (CST #9149) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nWestern Blotting: 1:1000\nImmunoprecipitation: 1:50\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting, Immunoprecipitation\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nSMG-1 (D42D5) Rabbit Monoclonal Antibody recognizes endogenous levels of total SMG-1 protein.\nSpecies Reactivity: Human, Monkey\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nMonoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human SMG-1 protein.\n\u003cb\u003eBackground\u003c\/b\u003e\nSMG-1 is a member of the phosphoinositide 3-kinase-related kinase (PIKK) family, which includes ATM, ATR, mTOR, DNA-PKcs, and TRRAP (1,2). Activated by DNA damage, SMG-1 has been shown to phosphorylate p53 and hUpf1 (SMG-2) (1-4). hUpf1 is a subunit of the surveillance complex that allows degradation of messenger RNA species containing premature termination codons (PTCs). This process, known as nonsense-mediated mRNA decay (NMD), prevents the translation of truncated forms of proteins that may result in gain of function or dominant negative species. NMD occurs under normal cellular conditions as well as in response to damage (5,6). SMG-1 has also been shown to affect cell death receptor signaling and to protect cells from extrinsically induced apoptotic cell death (7).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\n61E3.4; ATX; hSMG-1; KIAA0421; Lambda-interacting protein; Lambda\/iota protein kinase C-interacting protein; LIP; phosphatidylinositol 3-kinase-related kinase; phosphatidylinositol 3-kinase-related protein kinase; PI-3-kinase-related kinase SMG-1; Serine\/threonine-protein kinase SMG1; SMG-1; smg-1 homolog, phosphatidylinositol 3-kinase-related kinase; SMG1; SMG1 homolog, phosphatidylinositol 3-kinase-related kinase (C. elegans); SMG1 nonsense mediated mRNA decay associated PI3K related kinase; SMG1 phosphatidylinositol 3-kinase-related kinase\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H Mk\nSENSITIVITY: Endogenous\nMW (kDa): 410\nSource\/Isotype: Rabbit IgG","brand":"CST","offers":[{"title":"Default Title","offer_id":46800006906025,"sku":"9149S","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-9149s","provider":"Iright","version":"1.0","type":"link"}