{"product_id":"cst-9160s","title":"CST,  9160S, MEK1\/2 Control Cell Extracts","description":"Cell Extract Kit for studying MEK2\/MEK1 in the research area.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nBoil for 3 minutes prior to use. Load 20 µl of phosphorylated and nonphosphorylated MEK1\/2 Control Cell Extracts per lane.\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in SDS Sample Buffer: 62.5 mM Tris-HCl (pH 6.8 at 25°C), 2% w\/v SDS, 10% glycerol, 50 mM DTT, 0.01% w\/v phenol red or bromophenol blue. Store at -20°C or at -80°C for long term storage.\n\u003cb\u003eBackground\u003c\/b\u003e\nMEK1 and MEK2, also called MAPK or Erk kinases, are dual-specificity protein kinases that function in a mitogen activated protein kinase cascade controlling cell growth and differentiation (1-3). Activation of MEK1 and MEK2 occurs through phosphorylation of two serine residues at positions 217 and 221, located in the activation loop of subdomain VIII, by Raf-like molecules. MEK1\/2 is activated by a wide variety of growth factors and cytokines and also by membrane depolarization and calcium influx (1-4). Constitutively active forms of MEK1\/2 are sufficient for the transformation of NIH\/3T3 cells or the differentiation of PC-12 cells (4). MEK activates p44 and p42 MAP kinase by phosphorylating both threonine and tyrosine residues at sites located within the activation loop of kinase subdomain VIII.\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nlysates; MAPKK-1; MAPKK-2; MEK; MEK-1; MEK-2","brand":"CST","offers":[{"title":"Default Title","offer_id":46800723509417,"sku":"9160S","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-9160s","provider":"Iright","version":"1.0","type":"link"}