{"product_id":"cst-9194s","title":"CST,  9194S, p44\/42 MAPK (Erk1\/2) Control Cell Extracts","description":"Cell Extract Kit for studying ERK1\/ERK2 in the research area.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nBoil for 3 minutes prior to use. Load 15 μl of nonphosphorylated and phosphorylated p44\/42 MAPK (Erk1\/2) Control Cell Extracts per lane.\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in SDS Sample Buffer : 62.5 mM Tris-HCl (pH 6.8 at 25°C), 2% w\/v SDS, 10% glycerol, 50 mM DTT, 0.01% w\/v bromophenol blue or phenol red. Store at -20°C or -80°C for long-term storage.\n\u003cb\u003eBackground\u003c\/b\u003e\nMitogen-activated protein kinases (MAPKs) are a widely conserved family of serine\/threonine protein kinases involved in many cellular programs, such as cell proliferation, differentiation, motility, and death. The p44\/42 MAPK (Erk1\/2) signaling pathway can be activated in response to a diverse range of extracellular stimuli, including mitogens, growth factors, and cytokines (1-3), and research investigators consider it an important target in the diagnosis and treatment of cancer (4). Upon stimulation, a sequential three-part protein kinase cascade is initiated, consisting of a MAP kinase kinase kinase (MAPKKK or MAP3K), a MAP kinase kinase (MAPKK or MAP2K), and a MAP kinase (MAPK). Multiple p44\/42 MAP3Ks have been identified, including members of the Raf family, as well as Mos and Tpl2\/COT. MEK1 and MEK2 are the primary MAPKKs in this pathway (5,6). MEK1 and MEK2 activate p44 and p42 through phosphorylation of activation loop residues Thr202\/Tyr204 and Thr185\/Tyr187, respectively. Several downstream targets of p44\/42 have been identified, including p90RSK (7) and the transcription factor Elk-1 (8,9). p44\/42 are negatively regulated by a family of dual-specificity (Thr\/Tyr) MAPK phosphatases, known as DUSPs or MKPs (10), along with MEK inhibitors, such as U0126 and PD98059.\n\u003cb\u003eAlternate Names\u003c\/b\u003e\ncontrol lysate; erk; erk1; Erk1\/2; erk2; jurkat; map kinase; mapk; negative control; p42; p44; p44\/42; phorbol ester; pma; positive control; tpa; u0126","brand":"CST","offers":[{"title":"Default Title","offer_id":46800723706025,"sku":"9194S","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-9194s","provider":"Iright","version":"1.0","type":"link"}