{"product_id":"cst-91952s","title":"CST,  91952S, Phospho-c-Jun (Ser63) (E6I7P) Rabbit Monoclonal Antibody","description":"Monoclonal Antibody for studying JUN (Ser63) phosphate. Validated for WB,IP,IF,F,ChIP,C\u0026amp;R. Available in 2 sizes. Highly specific and rigorously validated in-house, Phospho-c-Jun (Ser63) (E6I7P) Rabbit Monoclonal Antibody (CST #91952) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nFor optimal ChIP results, use 10 μl of antibody and 10 μg of chromatin (approximately 4 x 106 cells) per IP. This antibody has been validated using SimpleChIP ® Enzymatic Chromatin IP Kits. The CUT\u0026amp;RUN dilution was determined using CUT\u0026amp;RUN Assay Kit # 86652 .\nWestern Blotting: 1:1000\nImmunoprecipitation: 1:50\nImmunofluorescence (Immunocytochemistry): 1:800 - 1:3200\nFlow Cytometry (Fixed\/Permeabilized): 1:100 - 1:200\nChromatin IP: 1:50\nCUT\u0026amp;RUN: 1:50\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 98248 .\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting, Immunoprecipitation, Immunofluorescence (Immunocytochemistry), Flow Cytometry (Fixed\/Permeabilized), Chromatin IP, CUT\u0026amp;RUN\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nPhospho-c-Jun (Ser63) (E6I7P) Rabbit Monoclonal Antibody recognizes endogenous levels of c-Jun protein only when phosphorylated at Ser63.\nSpecies Reactivity: Human, Mouse, Rat\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nMonoclonal antibody is produced by immunizing animals with a synthetic phospho-peptide corresponding to residues surrounding Ser63 of human c-Jun protein.\n\u003cb\u003eBackground\u003c\/b\u003e\nc-Jun is a member of the Jun family containing c-Jun, JunB, and JunD, and is a component of the transcription factor activator protein-1 (AP-1). AP-1 is composed of dimers of Fos, Jun, and ATF family members and binds to and activates transcription at TRE\/AP-1 elements (reviewed in 1). Extracellular signals, including growth factors, chemokines, and stress, activate AP-1-dependent transcription. The transcriptional activity of c-Jun is regulated by phosphorylation at Ser63 and Ser73 through SAPK\/JNK (reviewed in 2). Knockout studies in mice have shown that c-Jun is essential for embryogenesis (3), and subsequent studies have demonstrated roles for c-Jun in various tissues and developmental processes, including axon regeneration (4), liver regeneration (5), and T cell development (6). AP-1 regulated genes exert diverse biological functions, including cell proliferation, differentiation, and apoptosis, as well as transformation, invasion and metastasis, depending on cell type and context (7-9). Other target genes regulate survival, as well as hypoxia and angiogenesis (8,10). Research studies have implicated c-Jun as a promising therapeutic target for cancer, vascular remodeling, acute inflammation, and rheumatoid arthritis (11,12).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nActivator protein 1; AP-1; AP1; c-Jun; cJUN; enhancer-binding protein AP1; JUN; Jun activation domain binding protein; jun oncogene; Jun proto-oncogene, AP-1 transcription factor subunit; p39; Proto-oncogene c-Jun; proto-oncogene cJun; Transcription factor AP-1; Transcription factor Jun; V-jun avian sarcoma virus 17 oncogene homolog; v-jun sarcoma virus 17 oncogene homolog\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H M R\nSENSITIVITY: Endogenous\nMW (kDa): 48\nSource\/Isotype: Rabbit IgG","brand":"CST","offers":[{"title":"Default Title","offer_id":46800010772649,"sku":"91952S","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-91952s","provider":"Iright","version":"1.0","type":"link"}