{"product_id":"cst-92108t","title":"CST,  92108T, PELO (F3V8O) Rabbit Monoclonal Antibody","description":"Monoclonal Antibody for studying PELO. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, PELO (F3V8O) Rabbit Monoclonal Antibody (CST #92108) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nWestern Blotting: 1:1000\nImmunoprecipitation: 1:50\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting, Immunoprecipitation\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nPELO (F3V8O) Rabbit Monoclonal Antibody recognizes endogenous levels of total PELO protein. This antibody detects a 120 kDa band of unknown origin.\nSpecies Reactivity: Human, Mouse, Rat, Monkey\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nMonoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding His244 of human PELO protein.\n\u003cb\u003eBackground\u003c\/b\u003e\nPELO, also known as Pelota or Dom34, along with its binding partner HBS1, is involved in several key mRNA quality control mechanisms (1). The PELO-HBS1 complex utilizes the ATPase ABCE1 as an ATPase to cause dissociation of stalled elongation complexes (3,4). PELO-HBS1 can cause mRNA degradation through two main pathways: the nonstop mRNA degradation pathway, where the mRNA lacks a stop codon, and the no-go mRNA degradation pathway, where the ribosome stalls due to secondary structures or damaged nucleotides (2-5). PELO has been shown to play a multifaceted role in viral infection, detecting single-stranded viral RNAs and targeting them for degradation, while also facilitating RNA turnover for more efficient viral replication (6,7). PELO has been implicated in protection from age-related disease phenotypes (8).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nCGI-17; PELO; pelota homolog; pelota homolog (Drosophila); pelota mRNA surveillance and ribosome rescue factor; PRO1770; Protein pelota homolog\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H M R Mk\nSENSITIVITY: Endogenous\nMW (kDa): 42\nSource\/Isotype: Rabbit IgG","brand":"CST","offers":[{"title":"Default Title","offer_id":46800012640425,"sku":"92108T","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-92108t","provider":"Iright","version":"1.0","type":"link"}