{"product_id":"cst-9213s","title":"CST,  9213S, p38 MAPK Control Cell Extracts","description":"Cell Extract Kit for studying p38-alpha MAPK mouse\/p38-beta MAPK mouse\/p38-gamma MAPK mouse in the research area.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nBoil for 3 minutes prior to use. Load 15 µl of phosphorylated and nonphosphorylated p38 MAPK Control Cell Extracts per lane.\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in SDS Sample Buffer: 62.5 mM Tris-HCl (pH 6.8 at 25°C), 2% w\/v SDS, 10% glycerol, 50 mM DTT, 0.01% w\/v phenol red or bromophenol blue. Store at -20°C or at -80°C for long term storage.\n\u003cb\u003eBackground\u003c\/b\u003e\np38 MAP kinase (MAPK), also called RK (1) or CSBP (2), is the mammalian orthologue of the yeast HOG kinase that participates in a signaling cascade controlling cellular responses to cytokines and stress (1-4). Four isoforms of p38 MAPK, p38α, β, γ (also known as Erk6 or SAPK3), and δ (also known as SAPK4) have been identified. Similar to the SAPK\/JNK pathway, p38 MAPK is activated by a variety of cellular stresses, including osmotic shock, inflammatory cytokines, lipopolysaccharide (LPS), UV light, and growth factors (1-5). MKK3, MKK6, and SEK activate p38 MAPK by phosphorylation at Thr180 and Tyr182. Activated p38 MAPK has been shown to phosphorylate and activate MAPKAP kinase 2 (3) and to phosphorylate the transcription factors ATF-2 (5), Max (6), and MEF2 (5-8). SB203580 (4-(4-fluorophenyl)-2-(4-methylsulfinylphenyl)-5-(4-pyridyl)-imidazole) is a selective inhibitor of p38 MAPK. This compound inhibits the activation of MAPKAPK-2 by p38 MAPK and subsequent phosphorylation of HSP27 (9). SB203580 inhibits p38 MAPK catalytic activity by binding to the ATP-binding pocket, but does not inhibit phosphorylation of p38 MAPK by upstream kinases (10).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nCSBP; HOGK; p38; p38-MAP-kinase; p38-MAPK; p38-α; p38-β; p38-γ; p38-δ; p38α; p38β; p38γ; p38δ; RK; SAPK-2","brand":"CST","offers":[{"title":"Default Title","offer_id":46800723738793,"sku":"9213S","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-9213s","provider":"Iright","version":"1.0","type":"link"}