{"product_id":"cst-92145s","title":"CST,  92145S, TTF-I Antibody","description":"Polyclonal Antibody for studying TTF1. Validated for Western Blotting. Highly specific and rigorously validated in-house, TTF-I Antibody (CST #92145) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nWestern Blotting: 1:1000\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody.\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nTTF-I Antibody recognizes endogenous levels of total TTF-I protein.\nSpecies Reactivity: Human, Mouse, Rat, Monkey\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nPolyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding His34 of human TTF-I protein. Antibodies are purified by peptide affinity chromatography.\n\u003cb\u003eBackground\u003c\/b\u003e\nTranscription termination factor I (TTF-I) is an essential protein that controls the RNA Polymerase I transcription of the rDNA genes. TTF-I binds to the Sal Box, a repetitive sequence element in the spacer region downstream of the 3' ends of the genes. Binding of TTF-I to this element can pause the elongation complex through the activity of chromatin remodeling complexes, such as NoRC (1-5). TTF-I also has an activating function by binding an upstream terminator element to induce chromatin remodeling (6). TTF-I localization can be controlled by key tumor suppressor ARF, which binds to the TTF-I nucleolar localization sequence, sending it to the nucleoplasm. This process drastically reduces rRNA synthesis (7). Furthermore, TTF-I can be ubiquitinylated by MDM2, which targets it for degradation (8). TTF-I has also been identified to play a role in HIV-1 replication (9).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nRNA polymerase I termination factor; Transcription termination factor 1; Transcription termination factor I; transcription termination factor, RNA polymerase I; TTF-1; TTF-I; TTF1\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H M R Mk\nSENSITIVITY: Endogenous\nMW (kDa): 135\nSOURCE: Rabbit","brand":"CST","offers":[{"title":"Default Title","offer_id":46800013230249,"sku":"92145S","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-92145s","provider":"Iright","version":"1.0","type":"link"}