{"product_id":"cst-92261sf","title":"CST,  92261SF, Legumain (D6S4H) Rabbit Monoclonal Antibody (BSA and Azide Free)","description":"Monoclonal Antibody for studying LGMN. Validated for Western Blotting,Immunohistochemistry (Paraffin). Highly specific and rigorously validated in-house, Legumain (D6S4H) Rabbit Monoclonal Antibody (BSA and Azide Free) (CST #92261) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nThis product is the carrier free version of product #93627. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol. This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT\u0026amp;RUN or CUT\u0026amp;Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us . Optimal dilutions\/concentrations should be determined by the end user. BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity.\n\u003cb\u003eFormulation\u003c\/b\u003e\nSupplied in 1X PBS (10 mM Na 2 HPO 4 , 3 mM KCl, 2 mM KH 2 PO 4 , and 140 mM NaCl (pH 7.8)). BSA and Azide Free. For standard formulation of this product see product # 93627\n\u003cb\u003eStorage\u003c\/b\u003e\nStore at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze\/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance.\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nLegumain (D6S4H) Rabbit Monoclonal Antibody (BSA and Azide Free) recognizes endogenous levels of total Legumain protein. Multiple bands represent posttranslational modification, including autoproteolytic cleavage, of Legumain.\nSpecies Reactivity: Human, Mouse, Rat\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nMonoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu251 of human Legumain protein.\n\u003cb\u003eBackground\u003c\/b\u003e\nLegumain (LGMN) is an asparaginyl endopeptidase that mediates lysosomal processing of antigen for class II MHC presentation. Under normal conditions, LGMN is mostly found in lysosomes. However, its expression level, cellular compartmentalization, and function may change under certain conditions, including cancer (1, 2, 3). In colorectal cancer, LGMN is upregulated and is found extracellularly and in the nucleus in both primary tumors and colorectal cancer cell lines (4, 5). Legumain forms homodimers and may be activated by posttranslational modifications, including autoproteolytic cleavage, as it moves from one cellular compartment to another (6).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nAEP; Asparaginyl endopeptidase; cysteine protease 1; Legumain; LGMN; LGMN1; Protease, cysteine 1; protease, cysteine, 1 (legumain); PRSC1\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H M R\nSENSITIVITY: Endogenous\nMW (kDa): 25, 35, 55\nSource\/Isotype: Rabbit IgG","brand":"CST","offers":[{"title":"Default Title","offer_id":46800014311593,"sku":"92261SF","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-92261sf","provider":"Iright","version":"1.0","type":"link"}