{"product_id":"cst-92284sf","title":"CST,  92284SF, CaMKII-alpha (6G9) Mouse Monoclonal Antibody (BSA and Azide Free)","description":"Monoclonal Antibody for studying CaMK2-alpha. Validated for WB,IHC,IF,IF. Highly specific and rigorously validated in-house, CaMKII-alpha (6G9) Mouse Monoclonal Antibody (BSA and Azide Free) (CST #92284) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nThis product is the carrier free version of product #50049. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol. This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT\u0026amp;RUN or CUT\u0026amp;Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us . Optimal dilutions\/concentrations should be determined by the end user. BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity.\n\u003cb\u003eFormulation\u003c\/b\u003e\nSupplied in 1X PBS (10 mM Na 2 HPO 4 , 3 mM KCl, 2 mM KH 2 PO 4 , and 140 mM NaCl (pH 7.8)). BSA and Azide Free. For standard formulation of this product see product # 50049\n\u003cb\u003eStorage\u003c\/b\u003e\nStore at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze\/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance.\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nCaMKII-alpha (6G9) Mouse Monoclonal Antibody (BSA and Azide Free) recognizes endogenous levels of total CaMKII-α protein. This antibody does not cross-react with CaMKII-β or CaMKII-δ proteins.\nSpecies Reactivity: Human, Mouse, Rat\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nMonoclonal antibody is produced by immunizing animals with a partially purified rat CaMKII-α protein.\n\u003cb\u003eBackground\u003c\/b\u003e\nCaMKII is an important member of the calcium\/calmodulin-activated protein kinase family, functioning in neural synaptic stimulation and T cell receptor signaling (1,2). CaMKII has catalytic and regulatory domains. Ca \/calmodulin binding to the CaMKII regulatory domain relieves autoinhibition and activates the kinase (3). The activated CaMKII further autophosphorylates at Thr286 to render the kinase constitutively active (3). The threonine phosphorylation state of CaMKII can be regulated through PP1\/PKA. PP1 (protein phosphatase 1) dephosphorylates phospho-CaMKII at Thr286. PKA (protein kinase A) prevents phospho-CaMKII (Thr286) dephosphorylation through an inhibitory effect on PP1 (4).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\ncalcium\/calmodulin dependent protein kinase II alpha; calcium\/calmodulin-dependent protein kinase (CaM kinase) II alpha; calcium\/calmodulin-dependent protein kinase II; calcium\/calmodulin-dependent protein kinase II alpha; calcium\/calmodulin-dependent protein kinase II alpha-B subunit; calcium\/calmodulin-dependent protein kinase type II alpha chain; Calcium\/calmodulin-dependent protein kinase type II subunit alpha; calmodulin-dependent kinase II; CaM kinase II alpha subunit; CaM kinase II subunit alpha; CaM-kinase II alpha chain; CaMK-II alpha subunit; CaMK-II subunit alpha; CAMK2A; CAMKA; CaMKII; CaMKIIalpha; CaMKIINalpha; KCC2A; KIAA0968; MRD53; MRT63\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H M R\nSENSITIVITY: Endogenous\nMW (kDa): 50\nSource\/Isotype: Mouse IgG1","brand":"CST","offers":[{"title":"Default Title","offer_id":46800014704809,"sku":"92284SF","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-92284sf","provider":"Iright","version":"1.0","type":"link"}