{"product_id":"cst-92921s","title":"CST,  92921S, LAMC1 Antibody","description":"Polyclonal Antibody for studying LAMC1. Validated for Western Blotting,Immunoprecipitation. Highly specific and rigorously validated in-house, LAMC1 Antibody (CST #92921) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nWestern Blotting: 1:1000\nImmunoprecipitation: 1:50\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody.\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting, Immunoprecipitation\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nLAMC1 Antibody recognizes endogenous levels of total LAMC1 protein.\nSpecies Reactivity: Human, Mouse, Rat\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nPolyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Asp1365 of human LAMC1 protein. Antibodies are purified by protein A and peptide affinity chromatography.\n\u003cb\u003eBackground\u003c\/b\u003e\nLaminins are a family of proteins important for maintaining cellular basement membrane (BM) structure and function (1). Laminins exist as heterotrimers of alpha (LAMA), beta (LAMB), and gamma (LAMC) chains. LAMC1 (laminin gamma-1) is a ubiquitously expressed gamma chain, and has three distinct functional domain structures. The N-terminal LN domain binds to specific alpha and beta chains to form a unique laminin heterotrimer. The LE domain mediates interactions with nidogens, while the C-terminal coil-coil fragment is necessary for agrin association. Collectively, these interactions are critical for stabilization and function of the BM (1). Genetic ablation of LAMC1 has demonstrated a critical role for this protein in embryo implantation, lung and kidney development, and neuronal Schwann cell myelination and regeneration, in addition to Trypanosome infection (2-5). Upregulation of LAMC1 has also been associated with tumor progression in multiple tumor types (6-9), possibly by creating a BM environment that is favorable for cancer cell metastasis and invasion.\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nLAMB2; LAMC1; Laminin B2 chain; laminin subunit gamma 1; Laminin subunit gamma-1; Laminin-1 subunit gamma; Laminin-10 subunit gamma; Laminin-11 subunit gamma; Laminin-2 subunit gamma; Laminin-3 subunit gamma; Laminin-4 subunit gamma; Laminin-6 subunit gamma; Laminin-7 subunit gamma; Laminin-8 subunit gamma; Laminin-9 subunit gamma; laminin, gamma 1 (formerly LAMB2); MGC87297; S-LAM gamma; S-laminin subunit gamma\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H M R\nSENSITIVITY: Endogenous\nMW (kDa): 220-250\nSOURCE: Rabbit","brand":"CST","offers":[{"title":"Default Title","offer_id":46800022339753,"sku":"92921S","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-92921s","provider":"Iright","version":"1.0","type":"link"}