{"product_id":"cst-9344s","title":"CST,  9344S, Phospho-p90RSK (Thr359\/Ser363) Antibody","description":"Polyclonal Antibody for studying RSK3 (Thr356\/Ser360) phosphate\/RSK1 (Thr359\/Ser363) phosphate. Validated for Western Blotting,Immunoprecipitation. Highly specific and rigorously validated in-house, Phospho-p90RSK (Thr359\/Ser363) Antibody (CST #9344) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nWestern Blotting: 1:1000\nImmunoprecipitation: 1:50\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody.\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting, Immunoprecipitation\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nPhospho-p90RSK (Thr359\/Ser363) Antibody detects endogenous levels of p90RSK when phosphorylated at Thr359\/Ser363, and singly phosphorylated at Thr359. This antibody does not recognize p90RSK phosphorylated at other sites. This antibody does not cross-react with phospho-RSK2 or -RSK3 at the homologous sites.\nSpecies Reactivity: Human, Mouse, Rat, Monkey\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nPolyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Thr359\/Ser363 of rat RSK1. Antibodies are purified by protein A and peptide affinity chromatography.\n\u003cb\u003eBackground\u003c\/b\u003e\nThe 90 kDa ribosomal S6 kinases (RSK1-4) are a family of widely expressed Ser\/Thr kinases characterized by two nonidentical, functional kinase domains (1) and a carboxy-terminal docking site for extracellular signal-regulated kinases (ERKs) (2). Several sites both within and outside of the RSK kinase domain, including Ser380, Thr359, Ser363, and Thr573, are important for kinase activation (3). RSK1-3 are activated via coordinated phosphorylation by MAPKs, autophosphorylation, and phosphoinositide-3-OH kinase (PI3K) in response to many growth factors, polypeptide hormones, and neurotransmitters (3).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\n90 kDa ribosomal protein S6 kinase 1; 90 kDa ribosomal protein S6 kinase 2; dJ590P13.1 (ribosomal protein S6 kinase, 90kD, polypeptide 1); HU-1; HU-2; KS6A1; KS6A2; MAP kinase-activated protein kinase 1a; MAP kinase-activated protein kinase 1c; MAPK-activated protein kinase 1a; MAPK-activated protein kinase 1c; MAPKAP kinase 1a; MAPKAP kinase 1c; MAPKAPK-1a; MAPKAPK-1c; MAPKAPK1; MAPKAPK1A; MAPKAPK1C; mitogen-activated protein kinase-activated protein kinase 1C; p90-RSK 1; p90-RSK 2; p90-RSK3; p90RSK; p90RSK1; p90RSK2; p90S6K; pp90RSK3; ribosomal protein S6 kinase A1; ribosomal protein S6 kinase A2; ribosomal protein S6 kinase alpha 1; ribosomal protein S6 kinase alpha 2; Ribosomal protein S6 kinase alpha-1; Ribosomal protein S6 kinase alpha-2; ribosomal protein S6 kinase, 90kDa, polypeptide 1; ribosomal protein S6 kinase, 90kDa, polypeptide 2; Ribosomal S6 kinase 1; Ribosomal S6 kinase 3; RPS6KA1; RPS6KA2; RSK; RSK-1; RSK-3; RSK1; RSK3; S6K-alpha; S6K-alpha 1; S6K-alpha-1; S6K-alpha-2; S6K-alpha2\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H M R Mk\nSENSITIVITY: Endogenous\nMW (kDa): 90\nSOURCE: Rabbit","brand":"CST","offers":[{"title":"Default Title","offer_id":46800026566825,"sku":"9344S","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-9344s","provider":"Iright","version":"1.0","type":"link"}