{"product_id":"cst-9389t","title":"CST,  9389T, SP1 (D4C3) Rabbit Monoclonal Antibody","description":"Monoclonal Antibody for studying SP1. Validated for WB,IP,IHC,F,ChIP,ChIP,C\u0026amp;R,C\u0026amp;T. Available in 2 sizes. Highly specific and rigorously validated in-house, SP1 (D4C3) Rabbit Monoclonal Antibody (CST #9389) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nFor optimal ChIP and ChIP-seq results, use 5 μl of antibody and 10 μg of chromatin (approximately 4 x 10 6 cells) per IP. This antibody has been validated using SimpleChIP ® Enzymatic Chromatin IP Kits. The CUT\u0026amp;RUN dilution was determined using CUT\u0026amp;RUN Assay Kit # 86652 . The CUT\u0026amp;Tag dilution was determined using CUT\u0026amp;Tag Assay Kit # 77552 .\nWestern Blotting: 1:1000\nImmunoprecipitation: 1:50\nImmunohistochemistry (Paraffin): 1:1000 - 1:4000\nFlow Cytometry (Fixed\/Permeabilized): 1:400 - 1:1600\nChromatin IP: 1:100\nChromatin IP-seq: 1:100\nCUT\u0026amp;RUN: 1:50\nCUT\u0026amp;Tag: 1:50\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 84386 .\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting, Immunoprecipitation, Immunohistochemistry (Paraffin), Flow Cytometry (Fixed\/Permeabilized), Chromatin IP, Chromatin IP-seq, CUT\u0026amp;RUN, CUT\u0026amp;Tag\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nSP1 (D4C3) Rabbit Monoclonal Antibody recognizes endogenous levels of total SP1 protein. This antibody is predicted to detect all three known isoforms.\nSpecies Reactivity: Human, Monkey\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nMonoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro593 of human SP1 protein (Isoform 1).\n\u003cb\u003eBackground\u003c\/b\u003e\nSpecificity protein 1 (SP1) is a ubiquitously expressed transcription factor belonging to the family of C2H2-type zinc finger containing DNA-binding proteins. SP1 binds GC-rich motifs with high affinity and regulates the expression of numerous mammalian genes (1,2). It interacts with many other transcription factors, such as c-Myc, EGR1, and Stat1, and with basal transcription machinery components. SP1 interacts with chromatin-modifying factors, such as histone deacetylases (HDACs) and p300 in chromatin remodeling. Transcriptional activity and stability of SP1 are regulated by post-translational modification, including phosphorylation, acetylation, ubiquitination, and glycosylation (3). Glycosylation of SP1 following insulin treatment leads to increased nuclear localization, while glucagon treatment increases cytoplasmic SP1 levels (4-6). Investigators have found high levels of SP1 in patients with Alzheimer's disease (7).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nSP1; Sp1 transcription factor; specificity protein 1; Transcription factor Sp1; TSFP1\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H Mk\nSENSITIVITY: Endogenous\nMW (kDa): 90\nSource\/Isotype: Rabbit IgG","brand":"CST","offers":[{"title":"Default Title","offer_id":46800030924969,"sku":"9389T","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-9389t","provider":"Iright","version":"1.0","type":"link"}