{"product_id":"cst-9391s","title":"CST,  9391S, Phospho-Threonine-Proline Mouse Monoclonal Antibody (P-Thr-Pro-101)","description":"Monoclonal Antibody for studying . Validated for Western Blotting,Immunohistochemistry (Paraffin),Peptide ELISA (DELFIA). Available in 2 sizes. Highly specific and rigorously validated in-house, Phospho-Threonine-Proline Mouse Monoclonal Antibody (P-Thr-Pro-101) (CST #9391) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nWestern Blotting: 1:5000\nImmunohistochemistry (Paraffin): 1:400\nPeptide ELISA (DELFIA): 1:1000\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting, Immunohistochemistry (Paraffin)\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nPhospho-Threonine-Proline Mouse Monoclonal Antibody (P-Thr-Pro-101) detects phospho-threonine only when followed by proline. This antibody reacts with proteins and peptides phosphorylated at the Thr-Pro motif in an otherwise highly context-independent fashion. This antibody is phospho-specific, but does not recognize phospho-threonine in the absence of an adjacent proline. This antibody does not react with phospho-tyrosine but does react with some phospho-serine peptides containing the phospho-serine-proline motif (e.g., phospho-Elk-1).\nSpecies Reactivity: All Species Expected\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nMonoclonal antibody is produced by immunizing animals with synthetic phospho-threonine-proline-containing peptides. This antibody is a mouse IgM clone and can be recognized by anti-mouse Ig (whole molecule) secondary antibody.\n\u003cb\u003eBackground\u003c\/b\u003e\nThe MAPK and CDK families of serine\/threonine protein kinases play important roles in cell signaling and cell cycle control. These kinases phosphorylate threonine or serine followed by a proline residue (1-6). To facilitate the study and discovery of new MAPK and CDK substrates, Cell Signaling Technology has developed antibodies that bind to phospho-threonine or phospho-serine followed by proline. As determined by ELISA using a wide variety of phospho-Thr-Pro peptides, Phospho-Threonine-Proline Monoclonal Antibody (P-Thr-Pro-101) recognizes the phospho-Thr-Pro motif in a highly context-independent fashion. It also interacts with a broad range of phospho-Thr-Pro-containing proteins as determined by western analysis of nocodazole-treated Jurkat cell extracts resolved on 2-D gels.\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: All\nSENSITIVITY: Endogenous\nSource\/Isotype: Mouse IgM","brand":"CST","offers":[{"title":"Default Title","offer_id":46800238215337,"sku":"9391S","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-9391s","provider":"Iright","version":"1.0","type":"link"}