{"product_id":"cst-9397s","title":"CST,  9397S, Phospho-CRMP-2 (Thr514) Antibody","description":"Polyclonal Antibody for studying CRMP-2 (Thr514) phosphate. Validated for Western Blotting. Highly specific and rigorously validated in-house, Phospho-CRMP-2 (Thr514) Antibody (CST #9397) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nWestern Blotting: 1:1000\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody.\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nPhospho-CRMP-2 (Thr514) Antibody detects endogenous levels of CRMP-2 only when phosphorylated at Thr514.\nSpecies Reactivity: Human, Mouse, Rat\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nPolyclonal antibodies are produced by immunizing animals with a synthetic peptide surrounding Thr514 of human CRMP-2. Antibodies are purified by peptide affinity chromatography.\n\u003cb\u003eBackground\u003c\/b\u003e\nCollapsin Response Mediator Protein-2 (CRMP-2) is expressed at high levels in the developing nervous system and plays a critical role in axonal outgrowth by specifying axon\/dendrite fate and establishing neuronal polarity (1,2). CRMP-2 enhances axon elongation and branching by binding to tubulin heterodimers to promote microtubule assembly (3). GSK-3β inactivates CRMP-2 by phosphorylating it at Thr514. CRMP-2 is primed following phosphorylation at Ser522 by CDK5 and at Thr518 by GSK-3β (2). Phosphorylation of CRMP-2, which decreases tubulin binding ability, can be inhibited by NT-3 and BDNF through the PI3 kinase\/Akt pathway (2). CRMP-2 also mediates semaphorin-induced growth cone collapse (4). Hyperphosphorylation of CRMP-2 is found in Alzheimer disease plaques with concurrent elevated GSK-3β activity in these patients (5).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nCollapsin response mediator protein 2; collapsin response mediator protein hCRMP-2; CRMP-2; CRMP2; DHPRP2; dihydropyrimidinase like 2; dihydropyrimidinase-like 2; dihydropyrimidinase-like 2 long form; Dihydropyrimidinase-related protein 2; DPYL2; DPYSL2; DRP-2; DRP2; N2A3; ULIP-2; ULIP2; Unc-33-like phosphoprotein 2\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H M R\nSENSITIVITY: Endogenous\nMW (kDa): 60-80\nSOURCE: Rabbit","brand":"CST","offers":[{"title":"Default Title","offer_id":46800031449257,"sku":"9397S","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-9397s","provider":"Iright","version":"1.0","type":"link"}