{"product_id":"cst-94101t","title":"CST,  94101T, IkappaB beta (D2O1T) Rabbit Monoclonal Antibody","description":"Monoclonal Antibody for studying IkB-beta mouse. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, IkappaB beta (D2O1T) Rabbit Monoclonal Antibody (CST #94101) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nWestern Blotting: 1:1000\nImmunoprecipitation: 1:100\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting, Immunoprecipitation\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nIkappaB beta (D2O1T) Rabbit Monoclonal Antibody recognizes endogenous levels of total rodent IκBβ protein.\nSpecies Reactivity: Mouse, Rat\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nMonoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Arg155 of mouse IκBβ protein.\n\u003cb\u003eBackground\u003c\/b\u003e\nThe NF-κB\/Rel transcription factors are present in the cytosol in an inactive state complexed with the inhibitory IκB proteins (1-3). Activation occurs via phosphorylation of IκBα at Ser32 and Ser36 followed by proteasome-mediated degradation that results in the release and nuclear translocation of active NF-κB (3-7). IκBα phosphorylation and resulting Rel-dependent transcription are activated by a highly diverse group of extracellular signals including inflammatory cytokines, growth factors, and chemokines. Kinases that phosphorylate IκB at these activating sites have been identified (8). The regulation of IκBβ and IκBε is similar to that of IκBα. However, the phosphorylation and ubiquitin-mediated degradation of these proteins occurs with much slower kinetics (9). IKK phosphorylation of IκBβ occurs at Ser19 and Ser23, while IκBε can be phosphorylated at Ser18 and Ser22 (10).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nI-kappa-B-beta; I(Kappa)B(beta); IKapp; IkappaBbeta; IkB; IkB-B; IkB-beta; Ikbb; MGC36057; NF-kappa-B inhibitor beta; NF-kappa-BIB; Nfkbib; nuclear factor of kappa light chain gene enhancer in B-cells inhibitor, beta; nuclear factor of kappa light polypeptide gene enhancer in B cells inhibitor, beta; nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, beta; OTTMUSP00000018308; OTTMUSP00000018309\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: M R\nSENSITIVITY: Endogenous\nMW (kDa): 48\nSource\/Isotype: Rabbit IgG","brand":"CST","offers":[{"title":"Default Title","offer_id":46800159113385,"sku":"94101T","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-94101t","provider":"Iright","version":"1.0","type":"link"}