{"product_id":"cst-94264sf","title":"CST,  94264SF, IgM (E9U8J) Rabbit Monoclonal Antibody (BSA and Azide Free)","description":"Monoclonal Antibody for studying IGHM. Validated for Western Blotting,IHC Leica Bond,Immunohistochemistry (Paraffin). Highly specific and rigorously validated in-house, IgM (E9U8J) Rabbit Monoclonal Antibody (BSA and Azide Free) (CST #94264) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nThis product is the carrier free version of product #77591. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol. This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT\u0026amp;RUN or CUT\u0026amp;Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us . Optimal dilutions\/concentrations should be determined by the end user. BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity.\n\u003cb\u003eFormulation\u003c\/b\u003e\nSupplied in 1X PBS (10 mM Na 2 HPO 4 , 3 mM KCl, 2 mM KH 2 PO 4 , and 140 mM NaCl (pH 7.8)). BSA and Azide Free. For standard formulation of this product see product # 77591\n\u003cb\u003eStorage\u003c\/b\u003e\nStore at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze\/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance.\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nIgM (E9U8J) Rabbit Monoclonal Antibody (BSA and Azide Free) recognizes endogenous levels of total IgM heavy chain protein. This antibody reacts with secreted and membrane bound isoforms of IgM heavy chain protein.\nSpecies Reactivity: Human\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nMonoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu310 within the constant region of human IgM heavy chain protein.\n\u003cb\u003eBackground\u003c\/b\u003e\nImmunoglobulin M (IgM) is one of the five major human immunoglobulin isotypes and is the first antibody to be secreted by plasmablasts in a humoral immune response after exposure to antigen. Structurally, IgM is the largest immunoglobulin and predominantly exists in pentameric form when secreted (1). Alternative splicing of the IgM heavy chain mRNA can generate an alternative form of the antibody, which facilitates its insertion into the plasma membrane of B cells to function in antigen recognition. IgM is the first Ig isotype to appear on the surface of developing B cells and is a major component of the B cell antigen receptor (BCR) signaling complex, which drives B cell survival and proliferation upon antigen-induced ligation (2,3). Research studies have shown that defects in the assembly of the BCR account for the low levels of surface IgM expression in B-chronic lymphocytic leukemia (4,5).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nAGM1; constant region of heavy chain of IgM; DKFZp686I15196; DKFZp686I15212; FLJ00385; Ig mu chain C region; Ig mu chain C region BOT; Ig mu chain C region GAL; Ig mu chain C region OU; IGHM; IgM; Immunoglobulin heavy constant mu; immunoglobulin mu chain; MGC104996; MGC52291; MU; VH\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H\nSENSITIVITY: Endogenous\nMW (kDa): 80\nSource\/Isotype: Rabbit IgG","brand":"CST","offers":[{"title":"Default Title","offer_id":46800033808553,"sku":"94264SF","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-94264sf","provider":"Iright","version":"1.0","type":"link"}