{"product_id":"cst-9429s","title":"CST,  9429S, Cleaved Caspase-8 (Asp387) Antibody","description":"Polyclonal Antibody for studying Casp8 (Asp387) cleaved mouse. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, Cleaved Caspase-8 (Asp387) Antibody (CST #9429) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nWestern Blotting: 1:1000\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody.\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nCleaved Caspase-8 (Asp387) Antibody detects endogenous levels of mouse and rat caspase-8 when cleaved at Asp387. This antibody will detect cleavage products containing the pro-domain with the p18 subunit (p43) as well as the p18 subunit alone.\nSpecies Reactivity: Mouse\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nPolyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues adjacent to the Asp387 cleavage site of rodent caspase-8. Antibodies were purified by protein A and peptide affinity chromatography.\n\u003cb\u003eBackground\u003c\/b\u003e\nApoptosis induced through the CD95 receptor (Fas\/APO-1) and tumor necrosis factor receptor 1 (TNFR1) activates caspase-8 and leads to the release of the caspase-8 active fragments, p18 and p10 (1-3). Activated caspase-8 cleaves and activates downstream effector caspases such as caspase-1, -3, -6, and -7. Caspase-3 ultimately elicits the morphological hallmarks of apoptosis, including DNA fragmentation and cell shrinkage.\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nCasp; CASP-8; Casp8; caspase 8; Caspase-8; Caspase-8 subunit p10; Caspase-8 subunit p18; caspase8; Fas-linked ICE-like protease; FLI; FLICE; MAC; MACH; Mch5\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: M\nSENSITIVITY: Endogenous\nMW (kDa): 18, 43\nSOURCE: Rabbit","brand":"CST","offers":[{"title":"Default Title","offer_id":46800159473833,"sku":"9429S","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-9429s","provider":"Iright","version":"1.0","type":"link"}