{"product_id":"cst-94346s","title":"CST,  94346S, CD22 (F9D1W) Rabbit Monoclonal Antibody","description":"Monoclonal Antibody for studying CD22 mouse. Validated for WB,IHC,IHC,IF. Available in 2 sizes. Highly specific and rigorously validated in-house, CD22 (F9D1W) Rabbit Monoclonal Antibody (CST #94346) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nWestern Blotting: 1:1000\nIHC Leica Bond: 1:50\nImmunohistochemistry (Paraffin): 1:50 - 1:200\nImmunofluorescence (Frozen): 1:1600 - 1:6400\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 47369 .\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting, IHC Leica Bond, Immunohistochemistry (Paraffin), Immunofluorescence (Frozen)\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nCD22 (F9D1W) Rabbit Monoclonal Antibody recognizes endogenous levels of total CD22 protein.\nSpecies Reactivity: Mouse\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nMonoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ser467 of mouse CD22 protein.\n\u003cb\u003eBackground\u003c\/b\u003e\nCD22 (also known as siglec-2) is a member of the sialic acid-binding immunoglobulin-type lectin (Siglec) family of immunomodulatory receptors. CD22 can bind to its ligand α 2,6-linked sialic acid on different cells (trans interaction) as well as on the same cells (cis interaction). CD22 is predominantly expressed on B cells and functions as an inhibitory co-receptor for the B cell receptor (BCR) (1,2). After BCR ligation, the tyrosine kinase Lyn is activated and phosphorylates two distal of the four ITIM motifs in the intracellular carboxy-terminal region of CD22, which then recruit tyrosine phosphatases, including SHP-1, to the plasma membrane, and in turn, they get tyrosine-phosphorylated and activated to damp the signaling pathways initiated by BCR ligation (3,4). CD22 has been actively pursued as a therapeutic target for autoimmune diseases (5). Due to almost exclusive expression on B cells, it is also actively pursued as a therapeutic target for multiple B cell malignancies (6-8).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nA530093D23; B-cell receptor CD22; B-lymphocyte cell adhesion molecule; BL-CAM; Cd22; CD22 antigen; Lyb; Lyb-; Lyb-8; Lyb8; Sialic acid-binding Ig-like lectin 2; Siglec-2; Siglec2; T-cell surface antigen Leu-14\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: M\nSENSITIVITY: Endogenous\nMW (kDa): 140\nSource\/Isotype: Rabbit IgG","brand":"CST","offers":[{"title":"Default Title","offer_id":46800159572137,"sku":"94346S","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-94346s","provider":"Iright","version":"1.0","type":"link"}