{"product_id":"cst-94350t","title":"CST,  94350T, NLK (D9X3C) Rabbit Monoclonal Antibody","description":"Monoclonal Antibody for studying NLK. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, NLK (D9X3C) Rabbit Monoclonal Antibody (CST #94350) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nWestern Blotting: 1:1000\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nNLK (D9X3C) Rabbit Monoclonal Antibody recognizes endogenous levels of total NLK protein.\nSpecies Reactivity: Human, Mouse, Rat\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nMonoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human NLK protein.\n\u003cb\u003eBackground\u003c\/b\u003e\nNemo-like kinase (NLK ) is a serine\/threonine-protein kinase that regulates multiple signaling pathways, including Wnt\/β-catenin, TGFβ, IL-6, and Notch (1-4). NLK contributes to cell proliferation, differentiation, cell fate determination during early embryogenesis and nervous system development in vertebrates (5-7). Recent studies showed that NLK is aberrantly expressed in various types of cancer where it regulates cancer cell proliferation, migration, invasion and survival (8-11). NLK is localized predominantly in nucleus and at a lower level in cytoplasm(12). Homodimerization of NLK is required for its activation and nuclear localization. NLK is activated via intermolecular autophosphorylation at Thr286 (13). NLK interacts with and phosphorylates a number of transcription factors including FOXO1, FOXO4, MYB, NOTCH1 and TCF7L2\/TCF4, and LEF-1\/TCF (14-18). NLK also associates with E3 ubiquitin ligase NARF and Raptor and regulates their function (19,20).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nDKFZp761G1211; FLJ21033; LAK1; nemo like kinase; Nemo-like kinase; NLK; Protein LAK1; Serine\/threonine-protein kinase NLK\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H M R\nSENSITIVITY: Endogenous\nMW (kDa): 58\nSource\/Isotype: Rabbit IgG","brand":"CST","offers":[{"title":"Default Title","offer_id":46800034889897,"sku":"94350T","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-94350t","provider":"Iright","version":"1.0","type":"link"}