{"product_id":"cst-94375s","title":"CST,  94375S, Phospho-IRF-3 (Ser396) (D6O1M) Rabbit Monoclonal Antibody (InTraSeq™ 3' Conjugate 3103)","description":"Monoclonal Antibody for studying IRF-3 (Ser396) phosphate. Validated for Single Cell Analysis - InTraSeq™ 3'. Highly specific and rigorously validated in-house, Phospho-IRF-3 (Ser396) (D6O1M) Rabbit Monoclonal Antibody (InTraSeq\u003csup\u003e™\u003c\/sup\u003e 3' Conjugate 3103) (CST #94375) is ready to ship.\n\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in PBS (pH 7.2), 2 mM EDTA, 0.05% Triton X-100, 2 mg\/mL BSA, and 50% glycerol. Store at -20°C. Do not aliquot the antibody .\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Single Cell Analysis - InTraSeq™ 3'\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nPhospho-IRF-3 (Ser396) (D6O1M) Rabbit mAb (InTraSeq™ 3' Conjugate 3103) recognizes endogenous levels of IRF-3 protein only when phosphorylated at Ser396.\nSpecies Reactivity: Human, Mouse\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nMonoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser396 of human IRF-3 protein.\n\u003cb\u003eBackground\u003c\/b\u003e\nInterferon regulatory factors (IRFs) comprise a family of transcription factors that function within the Jak\/Stat pathway to regulate interferon (IFN) and IFN-inducible gene expression in response to viral infection (1). IRFs play an important role in pathogen defense, autoimmunity, lymphocyte development, cell growth, and susceptibility to transformation. The IRF family includes nine members: IRF-1, IRF-2, IRF-9\/ISGF3γ, IRF-3, IRF-4 (Pip\/LSIRF\/ICSAT), IRF-5, IRF-6, IRF-7, and IRF-8\/ICSBP. All IRF proteins share homology in their amino-terminal DNA-binding domains. IRF family members regulate transcription through interactions with proteins that share similar DNA-binding motifs, such as IFN-stimulated response elements (ISRE), IFN consensus sequences (ICS), and IFN regulatory elements (IRF-E) (2). IRF-3 can inhibit cell growth and plays a critical role in controlling the expression of genes in the innate immune response (1-4). In unstimulated cells, IRF-3 is present in the cytoplasm. Viral infection results in phosphorylation of IRF-3 and leads to its translocation to the nucleus where it activates promoters containing IRF-3-binding sites. Phosphorylation of IRF-3 occurs at a cluster of C-terminal serine and threonine residues (between 385 and 405) leading to its association with the p300\/CBP coactivator protein that promotes DNA binding and transcriptional activity (5). During infection, IRF-3 is likely activated through a pathway that includes activation of Toll-like receptors and of a kinase complex that includes IKKε and TBK1 (6,7). IRF-3 is phosphorylated at Ser396 following viral infection, expression of viral nucleocapsid, and double stranded RNA treatment. These events likely play a role in the activation of IRF-3 (8).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nIIAE7; Interferon regulatory factor 3; IRF-3; IRF3\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H M\nSENSITIVITY: Endogenous\nSource\/Isotype: Rabbit IgG","brand":"CST","offers":[{"title":"Default Title","offer_id":46800780329129,"sku":"94375S","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-94375s","provider":"Iright","version":"1.0","type":"link"}