{"product_id":"cst-94449s","title":"CST,  94449S, Jagged1 (D4Y1R) Rabbit Monoclonal Antibody (PE Conjugate)","description":"Monoclonal Antibody for studying Jagged1. Validated for Flow Cytometry (Fixed\/Permeabilized). Highly specific and rigorously validated in-house, Jagged1 (D4Y1R) Rabbit Monoclonal Antibody (PE Conjugate) (CST #94449) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nFlow Cytometry (Fixed\/Permeabilized): 1:50\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in PBS (pH 7.2), less than 0.1% sodium azide and 2 mg\/ml BSA. Store at 4°C. Do not aliquot the antibody. Protect from light. Do not freeze.\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Flow Cytometry (Fixed\/Permeabilized)\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nJagged1 (D4Y1R) Rabbit Monoclonal Antibody (PE Conjugate) recognizes endogenous levels of total Jagged1 protein. This antibody conjugate does not cross-react with Jagged2 protein.\nSpecies Reactivity: Human, Mouse, Monkey\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nMonoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala1131 of human Jagged1 protein.\n\u003cb\u003eBackground\u003c\/b\u003e\nNotch signaling is activated upon engagement of the Notch receptor with its ligands, the DSL (Delta, Serrate, Lag2) single-pass type I membrane proteins. The DSL proteins contain multiple EGF-like repeats and a DSL domain that is required for binding to Notch (1,2). Five DSL proteins have been identified in mammals: Jagged1, Jagged2, Delta-like (DLL) 1, 3, and 4 (3). Ligand binding to the Notch receptor results in two sequential proteolytic cleavages of the receptor by the ADAM protease and the γ-secretase complex. The intracellular domain of Notch is released and then translocates to the nucleus, where it activates transcription. Notch ligands may also be processed in a way similar to Notch, suggesting a bi-directional signaling through receptor-ligand interactions (4-6).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nAGS; AGS1; AHD; AWS; CD339; DCHE; hJ1; JAG1; jagged 1 (Alagille syndrome); jagged canonical Notch ligand 1; Jagged1; JAGL1; MGC104644; Protein jagged-1\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H M Mk\nSENSITIVITY: Endogenous\nSource\/Isotype: Rabbit IgG","brand":"CST","offers":[{"title":"Default Title","offer_id":46800562061481,"sku":"94449S","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-94449s","provider":"Iright","version":"1.0","type":"link"}