{"product_id":"cst-9480s","title":"CST,  9480S, RagC (D8H5) Rabbit Monoclonal Antibody","description":"Monoclonal Antibody for studying RagC. Validated for WB,IP,IF,F. Available in 2 sizes. Highly specific and rigorously validated in-house, RagC (D8H5) Rabbit Monoclonal Antibody (CST #9480) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nWestern Blotting: 1:1000\nImmunoprecipitation: 1:50\nImmunofluorescence (Immunocytochemistry): 1:50 - 1:200\nFlow Cytometry (Fixed\/Permeabilized): 1:200 - 1:800\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 34037 .\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting, Immunoprecipitation, Immunofluorescence (Immunocytochemistry), Flow Cytometry (Fixed\/Permeabilized)\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nRagC (D8H5) Rabbit Monoclonal Antibody detects endogenous levels of total RagC protein.\nSpecies Reactivity: Human, Mouse, Rat, Monkey\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nMonoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues in the amino terminus of human RagC.\n\u003cb\u003eBackground\u003c\/b\u003e\nThe mTORC1 kinase complex is a critical regulator of cell growth (1,2). Its activity is modulated by energy levels, growth factors, and amino acids via signaling through Akt, MAPK, and AMPK pathways (3,4). Recent studies found that the four related GTPases, RagA, RagB, RagC, and RagD, interact with raptor within the mTORC1 complex (1,2). These interactions are both necessary and sufficient for mTORC1 activation in response to amino acid signals (1,2).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nFLJ13311; GTPase-interacting protein 2; GTR2; Rag C; Rag C protein; RagC; Ras related GTP binding C; Ras-related GTP binding C; Ras-related GTP-binding protein C; RRAGC; TIB929\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H M R Mk\nSENSITIVITY: Endogenous\nMW (kDa): 50\nSource\/Isotype: Rabbit IgG","brand":"CST","offers":[{"title":"Default Title","offer_id":46800040722601,"sku":"9480S","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-9480s","provider":"Iright","version":"1.0","type":"link"}