{"product_id":"cst-9491t","title":"CST,  9491T, Cleaved Caspase-7 (Asp198) Antibody","description":"Polyclonal Antibody for studying Casp7 (Asp198, 20kD) cleaved. Validated for Western Blotting,Simple Western™,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, Cleaved Caspase-7 (Asp198) Antibody (CST #9491) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nWestern Blotting: 1:1000\nSimple Western™: 1:10 - 1:50\nImmunoprecipitation: 1:100\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody.\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting, Immunoprecipitation\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nCleaved Caspase-7 (Asp198) Antibody detects endogenous levels of the large fragment of caspase-7 resulting from cleavage at aspartic acid 198. This antibody does not cross-react with full length caspase-7 or with other caspases.\nSpecies Reactivity: Human, Mouse, Rat, Monkey\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nPolyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to amino-terminal residues surrounding Asp198 in human caspase-7. Antibodies are purified by protein A and peptide affinity chromatography.\n\u003cb\u003eBackground\u003c\/b\u003e\nCaspase-7 (CMH-1, Mch3, ICE-LAP3) has been identified as a major contributor to the execution of apoptosis (1-4). Caspase-7, like caspase-3, is an effector caspase that is responsible for cleaving downstream substrates such as (ADP-ribose) polymerase and PARP (1,3). During apoptosis, caspase-7 is activated through proteolytic processing by upstream caspases at Asp23, Asp198, and Asp206 to produce the mature subunits (1,3). Similar to caspase-2 and -3, caspase-7 preferentially cleaves substrates following the recognition sequence DEVD (5).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nApoptotic protease Mch-3; CASP-7; CASP7; caspase 7; caspase 7 isoform delta; caspase 7, apoptosis-related cysteine peptidase; caspase 7, apoptosis-related cysteine protease; Caspase-7; Caspase-7 subunit p11; Caspase-7 subunit p20; caspase7; CMH-1; ICE-LAP3; ICE-like apoptotic protease 3; LICE2; Lice2 alpha\/beta\/gamma; MCH3\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H M R Mk\nSENSITIVITY: Endogenous\nMW (kDa): 20\nSOURCE: Rabbit","brand":"CST","offers":[{"title":"Default Title","offer_id":46800041377961,"sku":"9491T","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-9491t","provider":"Iright","version":"1.0","type":"link"}