{"product_id":"cst-9509t","title":"CST,  9509T, Cleaved Caspase-9 (Asp353) Antibody","description":"Polyclonal Antibody for studying Casp9 (Asp353) cleaved mouse. Validated for Western Blotting,Immunofluorescence (Immunocytochemistry). Available in 2 sizes. Highly specific and rigorously validated in-house, Cleaved Caspase-9 (Asp353) Antibody (CST #9509) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nWestern Blotting: 1:1000\nImmunofluorescence (Immunocytochemistry): 1:50\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody.\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting, Immunofluorescence (Immunocytochemistry)\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nCleaved Caspase-9 (Asp353) Antibody detects endogenous levels of the 37kDa subunit of mouse caspase-9 only after after cleavage at aspartic acid 353. This antibody does not cross-react with full length caspase-9 or with other caspases at endogenous levels. Non-specific proteins that are induced by apoptosis under certain conditions may be detected.\nSpecies Reactivity: Mouse\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nPolyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Asp353 of mouse caspase-9. Antibodies are purified by protein A and peptide affinity chromatography.\n\u003cb\u003eBackground\u003c\/b\u003e\nCaspase-9 (ICE-LAP6, Mch6) is an important member of the cysteine aspartic acid protease (caspase) family (1,2). Upon apoptotic stimulation, cytochrome c released from mitochondria associates with the 47 kDa procaspase-9\/Apaf-1. Apaf-1 mediated activation of caspase-9 involves intrinsic proteolytic processing, resulting in cleavage at Asp315 and producing a p35 subunit. Another cleavage occurs at Asp330, producing a p37 subunit that can serve to amplify the apoptotic response (3-6). Cleaved caspase-9 further processes other caspase members, including caspase-3 and caspase-7, to initiate a caspase cascade, which leads to apoptosis (7-10).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nAI115399; APAF-3; Apoptotic protease Mch-6; Apoptotic protease-activating factor 3; AW493809; Casp; CASP-9; Casp9; caspase 9; Caspase-9; Caspase-9 subunit p10; Caspase-9 subunit p35; Caspase9; ICE-; ICE-LAP6; ICE-like apoptotic protease 6; Mch6; OTTMUSP00000010525\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: M\nSENSITIVITY: Endogenous\nMW (kDa): 37\nSOURCE: Rabbit","brand":"CST","offers":[{"title":"Default Title","offer_id":46800161865897,"sku":"9509T","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-9509t","provider":"Iright","version":"1.0","type":"link"}