{"product_id":"cst-9517t","title":"CST,  9517T, SMAD5 Antibody","description":"Polyclonal Antibody for studying Smad5. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, SMAD5 Antibody (CST #9517) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nWestern Blotting: 1:1000\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody.\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nSMAD5 Antibody detects endogenous levels of total SMAD5 protein.\nSpecies Reactivity: Human, Rat, Monkey\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nPolyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to the residues surrounding Ile251 of human SMAD5. Antibodies are purified by protein A and peptide affinity chromatography.\n\u003cb\u003eBackground\u003c\/b\u003e\nBone morphogenetic proteins (BMPs) constitute a large family of signaling molecules that regulate a wide range of critical processes including morphogenesis, cell-fate determination, proliferation, differentiation, and apoptosis (1,2). BMP receptors are members of the TGF-β superfamily of Ser\/Thr kinase receptors. Ligand binding induces multimerization, autophosphorylation, and activation of these receptors (3-5). They subsequently phosphorylate SMAD1 at Ser463 and Ser465 in the carboxy-terminal motif SSXS, as well as SMAD5 and SMAD9 (SMAD8) at their corresponding sites. These phosphorylated SMADs dimerize with the coactivating SMAD4 and translocate to the nucleus, where they regulate the transcription of target genes (5). MAP kinases and CDKs 8 and 9 are also reported to phosphorylate residues in the linker region of SMAD1, including Ser206. Phosphorylation of SMAD1 at Ser206 recruits Smurf1 to the linker region and leads to the degradation of SMAD1 (6). Phosphorylation at this site also promotes SMAD1 transcriptional activity by recruiting YAP to the linker region (7).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nDKFZp781C1895; DKFZp781O1323; Dwfc; hSmad5; JV5-1; MAD homolog 5; MAD, mothers against decapentaplegic homolog 5; MADH5; Mothers against decapentaplegic homolog 5; mothers against decapentaplegic, drosophila, homolog of, 5; Mothers against DPP homolog 5; SMA- and MAD-related protein 5; SMAD 5; SMAD family member 5; SMAD, mothers against DPP homolog 5; SMAD5\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H R Mk\nSENSITIVITY: Endogenous\nMW (kDa): 60\nSOURCE: Rabbit","brand":"CST","offers":[{"title":"Default Title","offer_id":46800043999401,"sku":"9517T","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-9517t","provider":"Iright","version":"1.0","type":"link"}