{"product_id":"cst-9535s","title":"CST,  9535S, PDCD4 (D29C6) Rabbit Monoclonal Antibody","description":"Monoclonal Antibody for studying PDCD4. Validated for WB,IP,IHC,IF,eCLIP. Available in 2 sizes. Highly specific and rigorously validated in-house, PDCD4 (D29C6) Rabbit Monoclonal Antibody (CST #9535) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nFor more information about the RBP-eCLIP service please visit Eclipsebio .\nWestern Blotting: 1:1000\nImmunoprecipitation: 1:50\nImmunohistochemistry (Paraffin): 1:200\nImmunofluorescence (Immunocytochemistry): 1:600\neCLIP: 1:200\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting, Immunoprecipitation, Immunohistochemistry (Paraffin), Immunofluorescence (Immunocytochemistry)\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nPDCD4 (D29C6) Rabbit Monoclonal Antibody detects endogenous levels of total PDCD4 protein.\nSpecies Reactivity: Human, Mouse, Rat, Monkey\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nMonoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to human PDCD4 protein.\n\u003cb\u003eBackground\u003c\/b\u003e\nPDCD4 (programmed cell death protein 4) was identified as a suppressor of neoplastic transformation (1). It interacts with eukaryotic initiation factors eIF4AI and eIF4AII (2). PDCD4 colocalizes with eIF4A in the cytoplasm and inhibits the activity of eIF4A as an ATP-dependent RNA helicase (2). PDCD4 inhibits cap-dependent translation (2). Upon stimulation by mitogens, PDCD4 is phosphorylated on Ser67 by S6K1 kinase and then degraded through the ubiquitin pathway leading to enhanced translation and cell growth (3).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nH731; MGC33046; MGC33047; Neoplastic transformation inhibitor protein; nuclear antigen H731; Nuclear antigen H731-like; PDCD4; programmed cell death 4; programmed cell death 4 (neoplastic transformation inhibitor); Programmed cell death protein 4; Protein 197\/15a\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H M R Mk\nSENSITIVITY: Endogenous\nMW (kDa): 60\nSource\/Isotype: Rabbit IgG","brand":"CST","offers":[{"title":"Default Title","offer_id":46800046227625,"sku":"9535S","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-9535s","provider":"Iright","version":"1.0","type":"link"}