{"product_id":"cst-95462t","title":"CST,  95462T, CD31 (PECAM-1) (F8M3S) Rabbit Monoclonal Antibody","description":"Monoclonal Antibody for studying PECAM-1. Validated for Western Blotting,Simple Western™,Immunohistochemistry (Paraffin). Available in 2 sizes. Highly specific and rigorously validated in-house, CD31 (PECAM-1) (F8M3S) Rabbit Monoclonal Antibody (CST #95462) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nWestern Blotting: 1:1000\nSimple Western™: 1:50 - 1:250\nImmunohistochemistry (Paraffin): 1:50 - 1:200\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 71397 .\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting, Immunohistochemistry (Paraffin)\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nCD31 (PECAM-1) (F8M3S) Rabbit Monoclonal Antibody recognizes endogenous levels of total CD31 (PECAM-1) protein. Non-specific staining was observed of human testis by immunohistochemistry.\nSpecies Reactivity: Human, Monkey\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nMonoclonal antibody is produced by immunizing animals with recombinant protein specific to the amino terminus of human CD31 (PECAM-1) protein.\n\u003cb\u003eBackground\u003c\/b\u003e\nCD31 (Platelet Endothelial Cell Adhesion Molecule-1: PECAM-1), a member of the Ig superfamily of cell adhesion molecules, is expressed by circulating platelets, monocytes, neutrophils, some T cells, and endothelial cells and modulates cell adhesion, endothelial cell migration, and angiogenesis (1). CD31 is phosphorylated on Tyr686 at the cytoplasmic carboxy-terminal tail upon various stimuli (e.g. mechanical or oxidative stress), presumably by Src family members (2). The tyrosine phosphorylation mediates associations with a number of SH2 domain-containing binding partners such as PI3 kinase, SHIP, PLCγ, and SHP-2. Thus, CD31 serves as a scaffold for various signaling molecules (3).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nadhesion molecule; CD31; CD31 antigen; CD31\/EndoCAM; EndoCAM; FLJ58394; GPIIA'; PECA1; PECAM-1; PECAM-1, CD31\/EndoCAM; PECAM1; platelet and endothelial cell adhesion molecule 1; Platelet endothelial cell adhesion molecule; platelet endothelial cell adhesion molecule-1; platelet\/endothelial cell adhesion molecule\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H Mk\nSENSITIVITY: Endogenous\nMW (kDa): 135\nSource\/Isotype: Rabbit IgG","brand":"CST","offers":[{"title":"Default Title","offer_id":46800047669417,"sku":"95462T","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-95462t","provider":"Iright","version":"1.0","type":"link"}