{"product_id":"cst-95536s","title":"CST,  95536S, SARS-CoV-2 Nsp9 Antibody","description":"Polyclonal Antibody for studying nsp9-pp1a-pp1ab SARS-CoV-2. Validated for Western Blotting. Highly specific and rigorously validated in-house, SARS-CoV-2 Nsp9 Antibody (CST #95536) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nWestern Blotting: 1:1000\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/mL BSA, and 50% glycerol. Store at -20°C. Do not aliquot the antibody.\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nSARS-CoV-2 Nsp9 Antibody recognizes endogenous levels of total SARS-CoV-2 Nsp9 protein in infected cells. In infected Vero-E6 cells, a band of unknown identity was observed at 60 kDa, and a cross-reactive band was observed in both mock and infected Vero-E6 lysates at 35 kDa.\nSpecies Reactivity: Virus\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nPolyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly93 of SARS-CoV-2 Nsp9 protein. Antibodies are purified by peptide affinity chromatography.\n\u003cb\u003eBackground\u003c\/b\u003e\nThe cause of the COVID-19 pandemic is a novel and highly pathogenic coronavirus, termed SARS-CoV-2 (severe acute respiratory syndrome coronavirus-2). SARS-CoV-2 is a member of the Coronaviridae family of viruses (1). The genome of SARS-CoV-2 is relatively large and encodes up to 29 open reading frames (ORFs). These include ORF1a and ORF1b (further processed into 16 non-structural proteins), 9 accessory proteins, and 4 canonical structural proteins: spike (S), envelope (E), membrane (M), and nucleocapsid (N) (2). Nsp9 is a non-structural protein that is derived from ORF1a and ORF1b (2). Although the function of Nsp9 is not completely understood, it is known to be an RNA-binding protein with a unique architecture that interacts with the viral polymerase holoenzyme complex. It is well conserved between coronaviruses and is thought to be essential for viral replication, thus making it a target of therapeutic development (3).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nnon-structural protein 9; non-structural protein 9 (4141-4253); nsp9; nsp9-pp1a-pp1ab; SARS-CoV-2 nsp9\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: Vir\nSENSITIVITY: Endogenous\nMW (kDa): 10\nSOURCE: Rabbit","brand":"CST","offers":[{"title":"Default Title","offer_id":46800245489833,"sku":"95536S","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-95536s","provider":"Iright","version":"1.0","type":"link"}