{"product_id":"cst-9570t","title":"CST,  9570T, Phospho-eNOS (Ser1177) (C9C3) Rabbit Monoclonal Antibody","description":"Monoclonal Antibody for studying eNOS (Ser1177) phosphate. Validated for Western Blotting,Immunoprecipitation. Available in 3 sizes. Highly specific and rigorously validated in-house, Phospho-eNOS (Ser1177) (C9C3) Rabbit Monoclonal Antibody (CST #9570) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nWestern Blotting: 1:1000\nImmunoprecipitation: 1:25\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 55112 .\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting, Immunoprecipitation\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nPhospho-eNOS (Ser1177) (C9C3) Rabbit Monoclonal Antibody detects endogenous levels of eNOS only when phosphorylated at Ser1177.\nSpecies Reactivity: Human, Bovine, Pig\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nMonoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser1177 of human eNOS.\n\u003cb\u003eBackground\u003c\/b\u003e\nEndothelial nitric-oxide synthase (eNOS) is an important enzyme in the cardiovascular system. It catalyzes the production of nitric oxide (NO), a key regulator of blood pressure, vascular remodeling, and angiogenesis (1,2). The activity of eNOS is regulated by phosphorylation at multiple sites. The two most thoroughly studied sites are the activation site Ser1177 and the inhibitory site Thr495 (3). Several protein kinases including Akt\/PKB, PKA, and AMPK activate eNOS by phosphorylating Ser1177 in response to various stimuli (4,5). In contrast, bradykinin and H O activate eNOS activity by promoting both Ser1177 phosphorylation and Thr495 dephosphorylation (6,7).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\ncNOS; Constitutive NOS; EC-NOS; ECNOS; Endothelial NOS; eNOS; Nitric oxide synthase 3; nitric oxide synthase 3 (endothelial cell); nitric oxide synthase 3 transcript variant eNOS-delta20; nitric oxide synthase 3 transcript variant eNOS-delta20-21; nitric oxide synthase 3 transcript variant eNOS-delta21; Nitric oxide synthase, endothelial; NOS type III; NOS3; NOSIII\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H B Pg\nSENSITIVITY: Endogenous\nMW (kDa): 140\nSource\/Isotype: Rabbit IgG","brand":"CST","offers":[{"title":"Default Title","offer_id":46800049668265,"sku":"9570T","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-9570t","provider":"Iright","version":"1.0","type":"link"}