{"product_id":"cst-9578s","title":"CST,  9578S, Cleaved Drosophila Dcp-1 (Asp215) Antibody","description":"Polyclonal Antibody for studying Dcp-1 (Asp215) cleaved Drosophila. Validated for Western Blotting,Immunofluorescence (Immunocytochemistry). Highly specific and rigorously validated in-house, Cleaved Drosophila Dcp-1 (Asp215) Antibody (CST #9578) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nWestern Blotting: 1:1000\nImmunofluorescence (Immunocytochemistry): 1:800\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody.\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting, Immunofluorescence (Immunocytochemistry)\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nCleaved Drosophila Dcp-1 (Asp215) Antibody recognizes endogenous levels of the large 22 kDa fragment of cleaved Dcp-1. This antibody does not recognize full length Dcp-1. This antibody also detects a non-specific, apoptotic-related band at 50 kDa by western blot.\nSpecies Reactivity: D. melanogaster\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nPolyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to amino-terminal residues adjacent to Asp215 of Drosophila Dcp-1 protein. Antibodies are purified by protein A and peptide affinity chromatography.\n\u003cb\u003eBackground\u003c\/b\u003e\nCell death in the fruit fly Drosophila melanogaster is regulated by many of the same stimuli as mammalian cell death (1). The Drosophila genome contains seven caspase genes; three encode initiator caspases, and four encode effector caspases (reviewed in (2)). The Drosophila effector caspase, death caspase-1 (Dcp-1), is a critical executioner of apoptosis. It is involved in the proteolytic cleavage of many key proteins, such as the nuclear enzyme poly (ADP-ribose) polymerase (PARP). The activation of Dcp-1 requires proteolytic processing of its inactive zymogen into active p22 and p13 fragments (3). Comparison of the in vivo activity between DrICE and Dcp-1 has shown that DrICE is a more effective inducer of apoptosis than Dcp-1, which instead plays a role in determining the rate of cell death (4).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nCASP7 iso2; Caspase-1; Caspase-1 subunit p13; Caspase-1 subunit p22; CG5370; Dcp-1; Dcp1\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: Dm\nSENSITIVITY: Endogenous\nMW (kDa): 22\nSOURCE: Rabbit","brand":"CST","offers":[{"title":"Default Title","offer_id":46800240574633,"sku":"9578S","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-9578s","provider":"Iright","version":"1.0","type":"link"}