{"product_id":"cst-9590s","title":"CST,  9590S, AMFR Antibody","description":"Polyclonal Antibody for studying AMFR. Validated for Western Blotting,Immunoprecipitation. Highly specific and rigorously validated in-house, AMFR Antibody (CST #9590) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nWestern Blotting: 1:1000\nImmunoprecipitation: 1:50\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody.\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting, Immunoprecipitation\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nAMFR Antibody recognizes endogenous levels of total AMFR protein. This antibody does not cross-react with HRD1.\nSpecies Reactivity: Human, Monkey, Dog\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nPolyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human AMFR protein. Antibodies are purified by protein A and peptide affinity chromatography.\n\u003cb\u003eBackground\u003c\/b\u003e\nAutocrine motility factor receptor (AMFR\/gp78) is a putative seven transmembrane domain G protein-coupled receptor that functions, in part, at the cell surface as a cytokine receptor for autocrine motility factor\/phosphoglucose isomerase (AMF\/PGI). AMFR is also localized to an intracellular mitochondria-associated smooth ER domain where it functions as an E3 ubiquitin ligase (1). AMFR function, as both a cytokine receptor and ubiquitin ligase, is linked to a variety of cellular signaling cascades associated with metastasis development and increased invasiveness. AMFR was initially proposed to be a RING-H2 E3 ubiquitin ligase after sequence analysis identified a catalytic RING finger and CUE motif, which are responsible for ubiquitin ligase activity and ubiquitin binding, respectively (2,3). Indeed, AMFR is a key component and amongst the best characterized ubiquitin ligases of the endoplasmic reticulum associated degradation (ERAD) machinery, a process involving recognition of misfolded proteins, ubiquitination, deglycosylation, retro-translocation to the cytosol, and targeting to the proteasome (4). Recent studies have shown that AMFR plays an important role in cholesterol homeostasis via the sterol-mediated ubiquitination of HMG-CoA reductase and its cofactor Insig-1 (5,6). Furthermore, AMFR has been implicated in the degradation of apolipoprotein B100 (7). It was recently reported that AMFR degrades the metastasis suppressor KAI-1\/CD-82, representing the first evidence that AMFR ubiquitin ligase activity is involved in metastasis development (8). Increased expression of AMFR correlates with a high incidence of recurrence and reduced survival in patients with bladder, colorectal, and gastric cancers (9-11).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nAMF receptor; AMF receptor, isoform 1; AMF receptor, isoform 2; AMFR; AMFR2; autocrine motility factor receptor; autocrine motility factor receptor, isoform 1; Autocrine motility factor receptor, isoform 2; E3 ubiquitin-protein ligase AMFR; gp78; RING finger protein 45; RING-type E3 ubiquitin transferase AMFR; RNF45\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H Mk Dg\nSENSITIVITY: Endogenous\nMW (kDa): 75\nSOURCE: Rabbit","brand":"CST","offers":[{"title":"Default Title","offer_id":46800051536041,"sku":"9590S","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-9590s","provider":"Iright","version":"1.0","type":"link"}