{"product_id":"cst-9614t","title":"CST,  9614T, Phospho-Akt Substrate (RXXS*\/T*) (110B7E) Rabbit Monoclonal Antibody","description":"Monoclonal Antibody for studying . Validated for WB,IP,IHC,E. Available in 3 sizes. Highly specific and rigorously validated in-house, Phospho-Akt Substrate (RXXS*\/T*) (110B7E) Rabbit Monoclonal Antibody (CST #9614) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nWestern Blotting: 1:1000\nImmunoprecipitation: 1:50\nImmunohistochemistry (Paraffin): 1:500 - 1:2000\nPeptide ELISA (DELFIA): 1:1000\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting, Immunoprecipitation, Immunohistochemistry (Paraffin)\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nPhospho-Akt Substrate (RXXS*\/T*) (110B7E) Rabbit Monoclonal Antibody recognizes peptides and proteins containing phospho-serine\/threonine preceded by arginine at the -3 position. There is some preference observed for peptides that contain phospho-serine\/threonine preceded by arginine at both positions -5 and -3.\nSpecies Reactivity: All Species Expected\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nMonoclonal antibody is produced by immunizing animals with synthetic phospho-Akt substrate peptides.\n\u003cb\u003eBackground\u003c\/b\u003e\nAn important class of kinases, referred to as Arg-directed kinases or AGC-family kinases, includes cAMP-dependent protein kinase (PKA), cGMP-dependent protein kinase (PKG), protein kinase C, Akt, and RSK. These kinases share a substrate specificity characterized by Arg at position -3 relative to the phosphorylated Ser or Thr (1,2). Akt plays a central role in mediating critical cellular responses including cell growth and survival, angiogenesis, and transcriptional regulation (3-5). While a number of Akt substrates are known (such as GSK-3, Bad, and caspase-9) many important substrates await discovery. Akt phosphorylates substrates only at Ser\/Thr in a conserved motif characterized by Arg at positions -5 and -3 (6). Phospho-Akt substrate-specific antibodies from Cell Signaling Technology are powerful tools for investigating the regulation of phosphorylation by Akt and other Arg-directed kinases, as well as for high throughput kinase drug discovery.\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: All\nSENSITIVITY: Endogenous\nSource\/Isotype: Rabbit IgG","brand":"CST","offers":[{"title":"Default Title","offer_id":46800239263913,"sku":"9614T","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-9614t","provider":"Iright","version":"1.0","type":"link"}