{"product_id":"cst-9621s","title":"CST,  9621S, Phospho-(Ser\/Thr) PKA Substrate Antibody","description":"Polyclonal Antibody for studying . Validated for WB,IP,IHC,E. Available in 2 sizes. Highly specific and rigorously validated in-house, Phospho-(Ser\/Thr) PKA Substrate Antibody (CST #9621) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nWestern Blotting: 1:1000\nImmunoprecipitation: 1:100\nImmunohistochemistry (Paraffin): 1:50 - 1:200\nPeptide ELISA (DELFIA): 1:1000\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody.\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting, Immunoprecipitation, Immunohistochemistry (Paraffin)\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nPhospho-(Ser\/Thr) PKA Substrate Antibody detects peptides and proteins containing a phospho-serine\/threonine residue with arginine at the -3 position. This antibody is a useful tool in identifying substrates of AGC family kinases, including PKA and PKC. This antibody does not cross-react with the nonphosphorylated PKA substrate motif.\nSpecies Reactivity: All Species Expected\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nPolyclonal antibodies are produced by immunizing animals with a synthetic phospho-PKA substrate peptide. Antibodies are purified by protein A and peptide affinity chromatography.\n\u003cb\u003eBackground\u003c\/b\u003e\nAn important class of kinases, refered to as Arg-directed kinases or AGC-family kinases, includes cAMP-dependent protein kinase (PKA), cGMP-dependent protein kinase (PKG), protein kinase C, Akt and RSK. These kinases share a substrate specificity characterized by Arg at position -3 relative to the phosphorylated Ser or Thr (1,2). Phospho-PKA substrate-specific antibodies from Cell Signaling Technology are powerful tools for investigating the regulation of phosphorylation by PKA and other Arg-directed kinases, as well as for high throughput kinase drug discovery.\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: All\nSENSITIVITY: Endogenous\nSOURCE: Rabbit","brand":"CST","offers":[{"title":"Default Title","offer_id":46800239100073,"sku":"9621S","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-9621s","provider":"Iright","version":"1.0","type":"link"}