{"product_id":"cst-9624s","title":"CST,  9624S, Phospho-PKA Substrate (RRXS*\/T*) (100G7E) Rabbit Monoclonal Antibody","description":"Monoclonal Antibody for studying . Validated for Western Blotting,Immunoprecipitation,Peptide ELISA (DELFIA). Available in 3 sizes. Highly specific and rigorously validated in-house, Phospho-PKA Substrate (RRXS*\/T*) (100G7E) Rabbit Monoclonal Antibody (CST #9624) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nWestern Blotting: 1:1000\nImmunoprecipitation: 1:50\nPeptide ELISA (DELFIA): 1:1000\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting, Immunoprecipitation\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nPhospho-PKA Substrate (RRXS*\/T*) (100G7E) Rabbit Monoclonal Antibody detects peptides and proteins containing a phospho-Ser\/Thr residue with arginine at the -3 and -2 positions. This antibody is a useful tool in identifying new substrates of PKA. This antibody recognizes other -3 arginine-bearing phospho-Ser\/Thr peptides, such as substrate motifs for Akt and PKC, to a lesser extent. This antibody does not recognize the nonphosphorylated substrate motif peptides.\nSpecies Reactivity: All Species Expected\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nMonoclonal antibody is produced by immunizing animals with synthetic phospho-PKA substrate peptides .\n\u003cb\u003eBackground\u003c\/b\u003e\nAn important class of kinases, refered to as Arg-directed kinases or AGC-family kinases, includes cAMP-dependent protein kinase (PKA), cGMP-dependent protein kinase (PKG), protein kinase C, Akt and RSK. These kinases share a substrate specificity characterized by Arg at position -3 relative to the phosphorylated Ser or Thr (1,2). Phospho-PKA substrate-specific antibodies from Cell Signaling Technology are powerful tools for investigating the regulation of phosphorylation by PKA and other Arg-directed kinases, as well as for high throughput kinase drug discovery.\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: All\nSENSITIVITY: Endogenous\nSource\/Isotype: Rabbit IgG","brand":"CST","offers":[{"title":"Default Title","offer_id":46800239329449,"sku":"9624S","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-9624s","provider":"Iright","version":"1.0","type":"link"}