{"product_id":"cst-9636s","title":"CST,  9636S, 14-3-3 beta\/alpha Antibody","description":"Polyclonal Antibody for studying 14-3-3 beta\/alpha. Validated for Western Blotting. Highly specific and rigorously validated in-house, 14-3-3 beta\/alpha Antibody (CST #9636) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nWestern Blotting: 1:1000\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody.\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\n14-3-3 beta\/alpha Antibody detects endogenous levels of total 14-3-3 β\/α protein.\nSpecies Reactivity: Human, Mouse, Rat, Monkey\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nPolyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Glu33 of human 14-3-3 β\/α protein. Antibodies are purified by protein A and peptide affinity chromatography.\n\u003cb\u003eBackground\u003c\/b\u003e\nThe 14-3-3 family of proteins plays a key regulatory role in signal transduction, checkpoint control, apoptotic and nutrient-sensing pathways (1,2). 14-3-3 proteins are highly conserved and ubiquitously expressed. There are at least seven isoforms, β, γ, ε, σ, ζ, τ, and η that have been identified in mammals. The initially described α and δ isoforms are confirmed to be phosphorylated forms of β and ζ, respectively (3). Through their amino-terminal α helical region, 14-3-3 proteins form homo- or heterodimers that interact with a wide variety of proteins: transcription factors, metabolic enzymes, cytoskeletal proteins, kinases, phosphatases, and other signaling molecules (3,4). The interaction of 14-3-3 proteins with their targets is primarily through a phospho-Ser\/Thr motif. However, binding to divergent phospho-Ser\/Thr motifs, as well as phosphorylation independent interactions has been observed (4). 14-3-3 binding masks specific sequences of the target protein, and therefore, modulates target protein localization, phosphorylation state, stability, and molecular interactions (1-4). 14-3-3 proteins may also induce target protein conformational changes that modify target protein function (4,5). Distinct temporal and spatial expression patterns of 14-3-3 isoforms have been observed in development and in acute response to extracellular signals and drugs, suggesting that 14-3-3 isoforms may perform different functions despite their sequence similarities (4). Several studies suggest that 14-3-3 isoforms are differentially regulated in cancer and neurological syndromes (2,3).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\n14-3-3 alpha; 14-3-3 beta; 14-3-3 protein beta\/alpha; 14-3-3 protein beta\/alpha, N-terminally processed; 1433B; brain protein 14-3-3, beta isoform; epididymis secretory protein Li 1; GW128; HEL-S-1; HS1; KCIP-1; Protein 1054; Protein kinase C inhibitor protein 1; protein kinase C inhibitor protein-1; tyrosine 3-monooxygenase\/tryptophan 5-monooxygenase activation protein beta; tyrosine 3-monooxygenase\/tryptophan 5-monooxygenase activation protein, alpha polypeptide; tyrosine 3-monooxygenase\/tryptophan 5-monooxygenase activation protein, beta polypeptide; YWHAA; YWHAB\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H M R Mk\nSENSITIVITY: Endogenous\nMW (kDa): 28\nSOURCE: Rabbit","brand":"CST","offers":[{"title":"Default Title","offer_id":46800054091945,"sku":"9636S","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-9636s","provider":"Iright","version":"1.0","type":"link"}