{"product_id":"cst-9647s","title":"CST,  9647S, RUNX3\/AML2 (D6E2) Rabbit Monoclonal Antibody","description":"Monoclonal Antibody for studying AML2. Validated for WB,IP,IHC,F. Available in 2 sizes. Highly specific and rigorously validated in-house, RUNX3\/AML2 (D6E2) Rabbit Monoclonal Antibody (CST #9647) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nWestern Blotting: 1:1000\nImmunoprecipitation: 1:50\nImmunohistochemistry (Paraffin): 1:50 - 1:200\nFlow Cytometry (Fixed\/Permeabilized): 1:100 - 1:400\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 86441 .\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting, Immunoprecipitation, Immunohistochemistry (Paraffin), Flow Cytometry (Fixed\/Permeabilized)\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nRUNX3\/AML2 (D6E2) Rabbit Monoclonal Antibody recognizes endogenous levels of both isoforms of RUNX3 protein. This antibody recognizes mouse RUNX3\/AML2 protein and is also reactive with human RUNX3\/AML2; however, this antibody is not suggested for immunohistochemical analysis of human tissues. This antibody does not recognize RUNX1 and RUNX2 proteins.\nSpecies Reactivity: Human, Mouse, Rat\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nMonoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human RUNX3 protein.\n\u003cb\u003eBackground\u003c\/b\u003e\nRunt-related transcription factor 3 (RUNX3, AML2), a member of the Runt family of transcription factors, plays an important role in the suppression of gastric epithelium cell proliferation (1), dorsal root ganglia neurogenesis (2), and T cell differentiation (3,4). RUNX3 is also involved in caspase-3-dependent apoptosis (5). Protein complexes containing RUNX3 and various transcription factors, such as Smads or β-catenin\/TCF4, have tumor suppressor activity and regulate downstream target gene transcription (6,7). While typically localized to the nucleus, RUNX3 can be tyrosine phosphorylated and located in the cytoplasm of many cancer cells. This mislocalization of RUNX3 abolishes its tumor suppressor function and contributes to tumorigenesis (8). Research studies indicate that gene silencing or protein mislocalization inactivates RUNX3 in more than 80% of gastric cancers and other cancer types (1,9,10).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nAcute myeloid leukemia 2 protein; acute myeloid leukemia gene 2; AML2; CBF-alpha-3; CBFA3; Core-binding factor subunit alpha-3; core-binding factor, runt domain, alpha subunit 3; FLJ34510; MGC16070; Oncogene AML-2; PEA2 alpha C; PEA2-alpha C; PEBP2 alpha C; PEBP2-alpha C; PEBP2A3; PEBP2aC; Polyomavirus enhancer-binding protein 2 alpha C subunit; runt related transcription factor 3; Runt-related transcription factor 3; RUNX family transcription factor 3; RUNX3; SL3-3 enhancer factor 1 alpha C subunit; SL3\/AKV core-binding factor alpha C subunit; transcription factor AML2\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H M R\nSENSITIVITY: Endogenous\nMW (kDa): 43-48\nSource\/Isotype: Rabbit IgG","brand":"CST","offers":[{"title":"Default Title","offer_id":46800055599273,"sku":"9647S","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-9647s","provider":"Iright","version":"1.0","type":"link"}