{"product_id":"cst-96703t","title":"CST,  96703T, SGPP1 (F2B6W) Rabbit Monoclonal Antibody","description":"Monoclonal Antibody for studying SGPP1. Validated for WB,WB,IP,F. Available in 2 sizes. Highly specific and rigorously validated in-house, SGPP1 (F2B6W) Rabbit Monoclonal Antibody (CST #96703) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nWestern Blotting: 1:1000\nSimple Western™: 1:10 - 1:50\nImmunoprecipitation: 1:100\nFlow Cytometry (Fixed\/Permeabilized): 1:50 - 1:200\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting, Immunoprecipitation, Flow Cytometry (Fixed\/Permeabilized)\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nSGPP1 (F2B6W) Rabbit Monoclonal Antibody recognizes endogenous levels of total SGPP1 protein.\nSpecies Reactivity: Human\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nMonoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala78 of human SGPP1 protein.\n\u003cb\u003eBackground\u003c\/b\u003e\nSphingosine kinases (SPHKs) catalyze the phosphorylation of sphingosine to form sphingosine-1-phosphate (S1P), a lipid mediator that affects various cellular functions, including immune response, neurogenesis, and angiogenesis (1). Sphingosine-1-phosphate phosphatase 1 (SGPP1) dephosphorylates S1P back to sphingosine, which is then readily converted to ceramide in the endoplasmic reticulum (ER) (2). The activity balance between SPHKs and SGPP1 is crucial for regulating sphingolipid metabolites and their downstream effects (3). SGPP1 overexpression results in elevated ceramide levels, which ultimately promotes apoptosis (4). Conversely, SGPP1 depletion increases S1P levels and induces autophagy and ER stress (5). Elevated S1P due to SGPP1 loss may also provide cellular resistance to cytotoxic agents, and SGPP1 has been found to be downregulated in gastric cancer and multiple myeloma (MM) (6-8).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nhSPP1; hSPPase1; SGPP1; Sphingosine-1-phosphatase 1; Sphingosine-1-phosphate phosphatase 1; Sphingosine-1-phosphate phosphohydrolase 1; SPP-1; Spp1; SPPase1\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H\nSENSITIVITY: Endogenous\nMW (kDa): 35\nSource\/Isotype: Rabbit IgG","brand":"CST","offers":[{"title":"Default Title","offer_id":46800057008297,"sku":"96703T","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-96703t","provider":"Iright","version":"1.0","type":"link"}